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The distribution and incidence of substance P (SP)- and Met-enkephalin-Arg6-Gly7-Leu8 (ENK-8)-like immunoreactive granule cells in the main and accessory olfactory bulbs of male rats was studied immunohistochemically. In the granule cell layer of the main olfactory bulb, numerous ENK-8-like immunoreactive granule cells were observed but SP-like immunoreactive ones were rare (less than 1%). On the other hand, in the granule cell layer of the accessory olfactory bulb, both SP-like immunoreactive and ENK-8-like immunoreactive granule cells were numerous. 15-20% of these neurons contained both the peptides.
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