-transparent.png){kind=logo}
The long-term effects of substance P (SP) depletion on epithelial integrity were assessed by measurements of airway transepithelial potential difference (PD) and resorptive clearance. Both techniques are highly sensitive to differences in epithelial permeability. PD (mV) was assayed with a KCl-saturated agar bridge technique in the lower trachea of Fischer 344 rats depleted of SP by neonatal capsaicin treatment. Capsaicin treatment is known to ablate a subpopulation of primary afferent fibers containing SP. Resorptive clearance (clearance in 1 h) was measured with 99mTc-labeled diethylenetriaminepentaacetic acid administered as a 100-microliters (100 microCi) bolus. Depletion of SP by neonatal treatment with capsaicin resulted in a highly significant increase in resorptive clearance (P less than 0.001). There was also a significant difference in PD in the lower trachea, the values being less negative for the capsaicin-treated rats (P less than 0.005). The increase in resorptive clearance may reflect the leakiness of the epithelial membrane, as also suggested by the decrease in PD. Aside from its known involvement as a neurotransmitter in neurogenic inflammation, SP may, under normal physiological conditions, have an additional role in the maintenance of epithelial barrier function.
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
February 2008,
Revue de pneumologie clinique,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
July 1985,
Thorax,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
April 1989,
Gastroenterology,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
March 1987,
Pediatric research,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
January 1985,
Gastroenterologie clinique et biologique,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
March 1970,
Comparative biochemistry and physiology,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
August 2004,
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
March 1991,
The Journal of pediatrics,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
March 1978,
The American journal of physiology,
G T De Sanctis, and
E M App, and
J K Trask, and
B I De Sanctis, and
J E Remmers, and
F H Green, and
S F Man, and
M King
January 1995,
Respiration physiology,
