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XR-1 is a Chinese hamster ovary (CHO) cell mutant which is unusually sensitive to killing by gamma rays in the G1 portion of the cell cycle but has nearly normal resistance to gamma-ray damage in late S phase. The cell-cycle sensitivity correlates with the mutant's inability to repair DNA double-strand breaks (DSBs) produced by ionizing radiation and restriction enzymes. We have previously shown in somatic cell hybrids of XR-1 cells and human fibroblasts that the XR-1 mutation is a recessive mutation. In this study, using somatic cell hybrids formed between XR-1 and human fibroblasts, we map the human complementing gene to chromosome 5 by chromosome-segregation analysis. This gene biochemically restores the hamster defect to wild-type levels of gamma-ray and bleomycin resistance as well as restoring its proficiency to repair DNA DSBs, suggesting that a single gene is responsible for the XR-1 phenotype. We have tentatively assigned the name XRCC4 (X-ray-complementing Chinese hamster gene 4) to this human gene until its biochemical function in repair is discovered.
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
September 2006,
DNA repair,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
August 1995,
Radiation research,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
June 2002,
DNA repair,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
October 1999,
Current biology : CB,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
April 2010,
The Journal of biological chemistry,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
January 2004,
Cytogenetic and genome research,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
April 2018,
Cold Spring Harbor protocols,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
August 1992,
Genomics,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
October 2001,
Seminars in radiation oncology,
A J Giaccia, and
N Denko, and
R MacLaren, and
D Mirman, and
C Waldren, and
I Hart, and
T D Stamato
December 2007,
Oncogene,
