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[Antibodies against hyaline cartilage. Antigen isolation and their characterization]. 1990

E Surmíková, and M Ruzicková
Oddelenia pre výskum histochémie a ultrastruktúrnej patológie Centra klinických oddelení VULB v Bratislave.

Extracts from human hyaline cartilage were analyzed quantitatively. The extracts were obtained by the action of salts, enzymatic processing and chromatography on Sephadex G-200 columns. The obtained fractions were characterized by thin layer chromatographic methods, electrophoretically, and immunochemically by means of polyclonal antibodies to individual components. Qualitative analysis of amino acids and electrophoretic separation showed that the 1st fraction contained mainly proteins and glycosaminoglycans after separation on Sephadex G-200, while the 2nd fraction exhibited mainly amino acids characteristic for collagen.

UI MeSH Term Description Entries
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D002356 Cartilage A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE. Cartilages
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006818 Hyalin A clear, homogenous, structureless, eosinophilic substance occurring in pathological degeneration of tissues. Hyalin Substance,Hyaline,Hyaline Substance,Hyalins,Substance, Hyalin,Substance, Hyaline
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D000941 Antigens Substances that are recognized by the immune system and induce an immune reaction. Antigen

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