Confirmatory assay increases specificity of the chlamydiazyme test for Chlamydia trachomatis infection of the cervix. 1990

J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
Department of Laboratory Medicine, University of California, San Francisco 94143.

Enzyme immunoassays for the detection of chlamydial antigens are commonly used to diagnose Chlamydia trachomatis infection. As is true for all nonculture methods, the specificities of these tests are a concern. A confirmatory blocking assay (Abbott Laboratories, North Chicago, Ill.) was evaluated at four sexually transmitted disease test sites. This assay is designed to confirm true-positive Chlamydiazyme (CZ) specimens and to identify false-positive CZ reactions caused by cross-reacting bacteria. Cervical specimens were collected from 2,891 women. Chlamydia prevalence by tissue culture (TC) was 9.2% (266 of 2,891 specimens). Compared with TC, the sensitivity and specificity of CZ were 78.9% (210 of 266 specimens) and 98.2% (2,577 of 2,625 specimens), respectively. There were 48 CZ false-positive reactions. The direct fluorescent-antibody test (DFA) was positive for 31 of 48 false-positive reactions, indicating culture misses. Thus, when the standard was both TC and DFA, CZ sensitivity was 81.1% and CZ specificity was 99.3%. Of the 17 CZ-positive patients who were negative by both TC and DFA, 3 were negative on repeat CZ and 11 of 14 were identified as false positive by the confirmatory assay. The confirmatory test was positive for CZ-positive women who were positive by TC or DFA. Use of the confirmatory test, which increased the specificity to 99.9%, would increase confidence in positive CZ results and make the test more useful for screening populations with a low prevalence of C. trachomatis infection.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D002575 Uterine Cervicitis Inflammation of the UTERINE CERVIX. Cervicitis,Cervicitides,Cervicitides, Uterine,Cervicitis, Uterine,Uterine Cervicitides
D002690 Chlamydia Infections Infections with bacteria of the genus CHLAMYDIA. Infections, Chlamydia,Chlamydia Infection,Infection, Chlamydia
D002692 Chlamydia trachomatis Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.
D003151 Community Health Centers Facilities which administer the delivery of health care services to people living in a community or neighborhood. Neighborhood Health Centers,Satellite Centers,Center, Community Health,Center, Neighborhood Health,Center, Satellite,Centers, Community Health,Centers, Neighborhood Health,Centers, Satellite,Community Health Center,Health Center, Community,Health Center, Neighborhood,Health Centers, Community,Health Centers, Neighborhood,Neighborhood Health Center,Satellite Center
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D005260 Female Females
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein

Related Publications

J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
March 1989, The Indian journal of medical research,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
December 1994, American journal of clinical pathology,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
January 1994, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
September 1984, Journal of clinical microbiology,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
February 1992, American journal of clinical pathology,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
November 1988, Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
December 1991, The Southeast Asian journal of tropical medicine and public health,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
November 1993, Ginecologia y obstetricia de Mexico,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
September 1991, Journal of clinical microbiology,
J Moncada, and J Schachter, and G Bolan, and J Engelman, and L Howard, and I Mushahwar, and G Ridgway, and G Mumtaz, and W Stamm, and A Clark
April 1986, Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
Copied contents to your clipboard!