Biomaterial Database

Combined effect of cadmium (CdCl2) and high temperature on HeLa S3 cells. 1990

T Kishimoto, and Y Fukuzawa, and M Tada

Department of Environmental Medicine, Shimane Medical University, Izumo, Japan.

The combined effect of cadmium (CdCl2) and high temperature on the subsequent growth, the synthesis of nucleic acids and protein, and the cell cycle in HeLa S3 cells was investigated. The subsequent growth rate was depressed by cadmium at 37.1 degrees C. This suppression effect was enhanced at 40.4 degrees C compared with the effect at 37.1 degrees C. The synthesis of DNA, RNA and protein was inhibited at higher temperatures in cultivation without cadmium, and depressed at each temperature in a concentration-dependent manner by the addition of cadmium. The DNA, RNA and protein-IC50 values at 40.4 degrees C decreased compared with the values at 37.1 degrees C. The DNA and RNA-IC50 values were significantly decreased (p less than 0.01, p less than 0.005, respectively) depending on the temperature. After treating the cells with cadmium at 40.4 degrees C, the DNA/BrdUrd distribution showed that the rate of dead cells increased and the rate of the G1/G0 phase decreased. These results indicate that the inhibitory effect of cadmium on the subsequent growth of HeLa S3 cells is enhanced at high temperature and this enhancement is related to the increased inhibitory effect of cadmium on DNA and RNA synthesis at high temperature.

UI	MeSH Term	Description	Entries
D002104	Cadmium	An element with atomic symbol Cd, atomic number 48, and atomic weight 112.41. It is a metal and ingestion will lead to CADMIUM POISONING.
D002106	Cadmium Radioisotopes	Unstable isotopes of cadmium that decay or disintegrate emitting radiation. Cd atoms with atomic weights 103-105, 107, 109, 115, and 117-119 are radioactive cadmium isotopes.	Radioisotopes, Cadmium
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012313	RNA	A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)	RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D014176	Protein Biosynthesis	The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.	Genetic Translation,Peptide Biosynthesis, Ribosomal,Protein Translation,Translation, Genetic,Protein Biosynthesis, Ribosomal,Protein Synthesis, Ribosomal,Ribosomal Peptide Biosynthesis,mRNA Translation,Biosynthesis, Protein,Biosynthesis, Ribosomal Peptide,Biosynthesis, Ribosomal Protein,Genetic Translations,Ribosomal Protein Biosynthesis,Ribosomal Protein Synthesis,Synthesis, Ribosomal Protein,Translation, Protein,Translation, mRNA,mRNA Translations