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OBJECTIVE To compare TK gene mutation assay in human lymphoblastoid cell lines TK6 and TK6-E6 induced by vinblastin and colcemid. METHODS Regular TK gene mutation assays were experimented to detect cytotoxicity and mutation frequency at tk locus after TK6 and TK6-E6 human lymphoblastoid cells were treated with vinblastin and colcemid for 24h. RESULTS Relative survival (RS%) and Relative suspension growth (RSG%) of TK6 and TK6-E6 cells decreased when concentrations of vinblastin and colcemid increased. RS% and RSG% of TK6-E6 cells were higher than that of TK6 cells at the same doses. TK6 and TK6-E6 cells both were induced by vinblastin and colcemid. The TK6-E6's nature mutation frequency, induced mutation frequency and percentage of slow growth mutant (SG%) were lower than TK6' s at the same dose except group CCM 5.0ng/ml in MF and group CCM 0.625ng/ml in SC%. CONCLUSIONS Both TK6 and TK6-E6 cell lines can be used in TK gene mutation assay, but TK6-E6 cell line was recommended to be used in the assay for its less quantity and doubling time.
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
April 2005,
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
May 2005,
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
March 2021,
Genes and environment : the official journal of the Japanese Environmental Mutagen Society,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
November 1981,
Cancer letters,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
December 1980,
Mutation research,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
January 1988,
Environmental and molecular mutagenesis,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
January 1988,
Environmental and molecular mutagenesis,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
January 1983,
Annals of the New York Academy of Sciences,
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
January 2019,
Methods in molecular biology (Clifton, N.J.),
Ya-nan Wang, and
Pei-qiang Shuai, and
Xiao-qin Ding, and
Li-shi Zhang
January 1989,
Environmental and molecular mutagenesis,
