BIOMAT Database Logo BIOMAT Database Logo

Nuclear import defect of human immunodeficiency virus type 1 DNA flap mutants is not dependent on the viral strain or target cell type. 2006

Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
Groupe de Virologie Moléculaire et Vectorologie, Département de Virologie, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris, France.

We have previously established, using human immunodeficiency virus type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear import. Although the impact of the DNA Flap on nuclear import has already found numerous independent confirmations in the context of lentivirus vectors, it has been claimed that it may be nonessential for infectious virus strains LAI, YU-2 (J. D. Dvorin et al., J. Virol. 76:12087-12096, 2002), HXB2, and NL4-3 (A. Limon et al., J. Virol. 76:12078-12086, 2002). We conducted a detailed analysis of virus infectivity using the provirus clones provided by the authors and analogous target cells. In contrast to published data, our results show that all cPPT mutant viruses exhibit reduced infectivity corresponding to a nuclear import defect irrespective of the viral genetic background or target cell.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001616 beta-Galactosidase A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. Lactases,Dairyaid,Lactaid,Lactogest,Lactrase,beta-D-Galactosidase,beta-Galactosidase A1,beta-Galactosidase A2,beta-Galactosidase A3,beta-Galactosidases,lac Z Protein,Protein, lac Z,beta D Galactosidase,beta Galactosidase,beta Galactosidase A1,beta Galactosidase A2,beta Galactosidase A3,beta Galactosidases
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D021581 Active Transport, Cell Nucleus Gated transport mechanisms by which proteins or RNA are moved across the NUCLEAR MEMBRANE. Nuclear Export,Nuclear Import,Nuclear Transport,Nucleocytoplasmic Transport,Nucleo-cytoplasmic Transport,Export, Nuclear,Import, Nuclear,Nucleo cytoplasmic Transport,Transport, Nuclear,Transport, Nucleo-cytoplasmic,Transport, Nucleocytoplasmic,Transports, Nucleo-cytoplasmic

Related Publications

Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
Phosphorylation-dependent human immunodeficiency virus type 1 infection and nuclear targeting of viral DNA.
January 1996, Proceedings of the National Academy of Sciences of the United States of America,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
Reassessment of the roles of integrase and the central DNA flap in human immunodeficiency virus type 1 nuclear import.
December 2002, Journal of virology,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
Nuclear import of human immunodeficiency virus type-1 preintegration complexes.
January 1999, Advances in virus research,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
The central DNA flap of the human immunodeficiency virus type 1 is important for viral replication.
October 2006, Biochemical and biophysical research communications,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
Human immunodeficiency virus type 1 KK26-27 matrix mutants display impaired infectivity, circularization and integration but not nuclear import.
August 2005, Virology,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
The karyophilic properties of human immunodeficiency virus type 1 integrase are not required for nuclear import of proviral DNA.
August 2000, Journal of virology,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
[Nuclear import of the genome from the human immunodeficiency virus type 1].
February 2006, Virologie (Montrouge, France),
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
Active nuclear import of human immunodeficiency virus type 1 preintegration complexes.
July 1992, Proceedings of the National Academy of Sciences of the United States of America,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
The human immunodeficiency virus type-1 central DNA flap is a crucial determinant for lentiviral vector nuclear import and gene transduction of human hematopoietic stem cells.
December 2000, Blood,
Nathalie Arhel, and Sandie Munier, and Philippe Souque, and Karine Mollier, and Pierre Charneau
The replicative impairment of Vif- mutants of human immunodeficiency virus type 1 correlates with an overall defect in viral DNA synthesis.
August 1998, The Journal of general virology,
Copied contents to your clipboard!