Biomaterial Database

Guanylyl cyclase is a heat-stable enterotoxin receptor. 1990

S Schulz, and C K Green, and P S Yuen, and D L Garbers

Howard Hughes Medical Institute, Vanderbilt University Medical Center, Nashville, Tennessee 37232.

Plasma membrane forms of guanylyl cyclase have been shown to function as natriuretic peptide receptors. We describe a new clone (GC-C) encoding a guanylyl cyclase receptor for heat-stable enterotoxin. GC-C encodes a protein containing an extracellular amino acid sequence divergent from that of previously cloned guanylyl cyclases; however, the protein retains the intracellular protein kinase-like and cyclase catalytic domains. Expression of GC-C in COS-7 cells results in high guanylyl cyclase activity. In addition, heat-stable enterotoxin from E. coli, but not natriuretic peptides, causes marked elevations of cyclic GMP and is specifically bound by cells transfected with GC-C. The enterotoxin fails to elevate cyclic GMP in nontransfected cells or in cells transfected with the natriuretic peptide/guanylyl cyclase receptors. These results show that a heat-stable enterotoxin receptor responsible for acute diarrhea is a plasma membrane form of guanylyl cyclase.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004768	Enterotoxins	Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.	Staphylococcal Enterotoxin,Enterotoxin,Staphylococcal Enterotoxins,Enterotoxin, Staphylococcal,Enterotoxins, Staphylococcal
D006162	Guanylate Cyclase	An enzyme that catalyzes the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. It also acts on ITP and dGTP. (From Enzyme Nomenclature, 1992) EC 4.6.1.2.	Guanyl Cyclase,Deoxyguanylate Cyclase,Guanylyl Cyclase,Inosinate Cyclase,Cyclase, Deoxyguanylate,Cyclase, Guanyl,Cyclase, Guanylate,Cyclase, Guanylyl,Cyclase, Inosinate
D000074261	Receptors, Enterotoxin	Guanylate cyclase-coupled receptors that bind bacterial ENTEROTOXINS, as well as the endogenous peptides guanylin and uroguanylin. Ligand binding stimulates production of CYCLIC GMP by EPITHELIAL CELLS of the intestinal lumen, altering barrier permeability and mucus secretion. Mutations in the gene (GUCY2C) encoding this receptor are associated with some cases of hereditary diarrhea (Diarrhea 6) and MECONIUM ILEUS.	Enterotoxin Receptor,Enterotoxin Receptors,Enterotoxin-Guanylin Receptor,Enterotoxin-Guanylin Receptors,GC-C Receptor,GC-C Receptors,Guanyl Cyclase-C Receptor,Guanyl Cyclase-C Receptors,Guanylin-Uroguanylin Receptor,Guanylin-Uroguanylin Receptors,Guanylyl Cyclase C,Heat-Stable Enterotoxin Receptor,Heat-Stable Enterotoxin Receptors,Receptor, Enterotoxin,Receptor, Guanyl Cyclase-C,Receptors, Guanyl Cyclase-C,Cyclase C, Guanylyl,Cyclase-C Receptors, Guanyl,Enterotoxin Guanylin Receptor,Enterotoxin Guanylin Receptors,Enterotoxin Receptor, Heat-Stable,Enterotoxin Receptors, Heat-Stable,GC C Receptor,GC C Receptors,Guanyl Cyclase C Receptor,Guanyl Cyclase C Receptors,Guanylin Uroguanylin Receptor,Guanylin Uroguanylin Receptors,Heat Stable Enterotoxin Receptor,Heat Stable Enterotoxin Receptors,Receptor, Enterotoxin-Guanylin,Receptor, GC-C,Receptor, Guanyl Cyclase C,Receptor, Guanylin-Uroguanylin,Receptor, Heat-Stable Enterotoxin,Receptors, Enterotoxin-Guanylin,Receptors, GC-C,Receptors, Guanyl Cyclase C,Receptors, Guanylin-Uroguanylin,Receptors, Heat-Stable Enterotoxin