Biomaterial Database

[Protective effects of enteral immuno-nutrition on intestinal mucosa injury in burned rats]. 2006

Chen Cai, and Guang-hua Guo, and Qing-lian Xu, and Yi-zhong Tang, and Sheng Liu

Department of Burns, First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China.

OBJECTIVE To investigate the protective effects of enteral immuno-nutrition on intestinal mucosa injury following severe burn. METHODS Sixty-four Wistar rats with 30% total body surface area (TBSA) full-thickness burn were randomly divided into enteral nutrition (EN, n=32) group and enteral immuno-nutrition (EIN, n=32) group. Another 8 Wistar rats were used as normal control group. The concentration of secretory immunoglobulin A (sIgA) in intestinal mucosa, the blood levels of diamine oxidase (DAO) activity and tumor necrosis factor-alpha (TNF-alpha) were determined, and the expression of TNF-alpha mRNA in intestinal mucosa was determined by reverse transcription polymerase chain reaction (PT-PCR) in control group and on 1st, 4th, 7th, and 10th day postburn in EN and EIN group. RESULTS Compared with that of before injury, the blood levels of DAO activity, TNF-alpha content, the concentration of sIgA and the expression of TNF-alpha mRNA in intestinal mucosa were significantly increased in both EN and EIN groups (P<0.05 or P<0.01). However, the blood levels DAO activity, TNF-alpha content, the concentration of sIgA on 4th, 7th and 10th postinjury days and the expression of TNF-alpha mRNA in intestinal mucosa on 1st, 4th, 7th and 10th days postinjury in EIN group were significantly lower than those in EN group (P<0.05 or P<0.01). CONCLUSIONS EIN can ameliorate the damage of the intestinal mucosa in burned rats compared with EN group. This might be related to an increase in the sIgA level and reduction of the expression of TNF-alpha mRNA in intestinal mucosa.

UI	MeSH Term	Description	Entries
D007070	Immunoglobulin A	Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.	IgA,IgA Antibody,IgA1,IgA2,Antibody, IgA
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D008297	Male		Males
D011897	Random Allocation	A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.	Randomization,Allocation, Random
D002056	Burns	Injuries to tissues caused by contact with heat, steam, chemicals (BURNS, CHEMICAL), electricity (BURNS, ELECTRIC), or the like.	Burn
D004195	Disease Models, Animal	Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases.	Animal Disease Model,Animal Disease Models,Disease Model, Animal
D004750	Enteral Nutrition	Nutritional support given via the alimentary canal or any route connected to the gastrointestinal system (i.e., the enteral route). This includes oral feeding, sip feeding, and tube feeding using nasogastric, gastrostomy, and jejunostomy tubes.	Enteral Feeding,Force Feeding,Nutrition, Enteral,Tube Feeding,Gastric Feeding Tubes,Feeding Tube, Gastric,Feeding Tubes, Gastric,Feeding, Enteral,Feeding, Force,Feeding, Tube,Feedings, Force,Force Feedings,Gastric Feeding Tube,Tube, Gastric Feeding,Tubes, Gastric Feeding
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012333	RNA, Messenger	RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.	Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated