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The use of solid-phase assays for the detection of irregular antibodies facilitates automatization and rationalization in blood group serology. We compared the solid screen assay, (SP-assay, Biotest AG, Frankfurt, FRG) with conventional assays for antibody screening. The SP assay was as sensitive as the conventional indirect antibody test (IAT). Whereas only 4% of cold-reactive antibodies detected in enzyme tests showed positive results in the SP-assay, this test produced unspecific positive reactions in 7% of all tested sera. We found two additional antibodies with the SP-assay, not detectable in conventional techniques (anti-D, immune anti-A).
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