BIOMAT Database Logo BIOMAT Database Logo

A human CD5+ B cell clone that secretes an idiotype-specific high affinity IgM monoclonal antibody. 1991

R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

We previously demonstrated the occurrence of a naturally arisen human anti-idiotypic B cell clone, that we transformed with EBV (EBV383). We show evidence that EBV383 not only expresses the CD5 surface Ag, but also contains the 2.7-kb mRNA transcript encoding this protein. In addition, we show the presence of the 3.6-kb mRNA precursor. Most Ig produced by CD5+ B cells are polyreactive natural IgM antibodies encoded by unmutated copies of germline VH genes. However, in this study we present data demonstrating the monoreactive high affinity character of the anti-idiotypic antibody (mAb383) produced by EBV383. These data are in agreement with our previous observations, showing that the VH chain of mAb383 is encoded by an extensive somatically mutated VHV gene in a way that is consistent with an Ag-driven immune response. A possible role for this remarkable anti-idiotypic antibody in the maintenance of B cell memory is discussed.

UI MeSH Term Description Entries
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007156 Immunologic Memory The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus. Immune Memory,Immunological Memory,Memory, Immunologic,Immune Memories,Immunologic Memories,Immunological Memories,Memory, Immune,Memory, Immunological
D002472 Cell Transformation, Viral An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus. Transformation, Viral Cell,Viral Cell Transformation,Cell Transformations, Viral,Transformations, Viral Cell,Viral Cell Transformations
D002999 Clone Cells A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed) Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D004854 Herpesvirus 4, Human The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies. Burkitt Herpesvirus,Burkitt Lymphoma Virus,E-B Virus,EBV,Epstein-Barr Virus,Human Herpesvirus 4,Infectious Mononucleosis Virus,Burkitt's Lymphoma Virus,HHV-4,Herpesvirus 4 (gamma), Human,Burkitts Lymphoma Virus,E B Virus,E-B Viruses,Epstein Barr Virus,Herpesvirus, Burkitt,Infectious Mononucleosis Viruses,Lymphoma Virus, Burkitt,Mononucleosis Virus, Infectious,Mononucleosis Viruses, Infectious
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000915 Antibody Affinity A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes. Affinity, Antibody,Antibody Avidity,Avidity, Antibody,Affinities, Antibody,Antibody Affinities,Antibody Avidities,Avidities, Antibody
D000943 Antigens, Differentiation Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation. Differentiation Antigen,Differentiation Antigens,Differentiation Antigens, Hairy Cell Leukemia,Differentiation Marker,Differentiation Markers,Leu Antigen,Leu Antigens,Marker Antigen,Marker Antigens,Markers, Differentiation,Antigen, Differentiation,Antigen, Leu,Antigen, Marker,Antigens, Leu,Antigens, Marker,Marker, Differentiation

Related Publications

R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Lupus-derived human monoclonal IgM anti-DNA antibody displays monospecificity, high affinity and private idiotype specificity.
December 1992, Lupus,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Characterization of a neoplastic B cell clone that secretes IgM in response to Th2-derived lymphokines.
January 1990, The Journal of molecular and cellular immunology : JMCI,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
A clone-specific monoclonal antibody that inhibits cytolysis of a cytolytic T cell clone.
March 1983, The Journal of experimental medicine,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Human rheumatoid B-1a (CD5+ B) cells make somatically hypermutated high affinity IgM rheumatoid factors.
July 1993, Journal of immunology (Baltimore, Md. : 1950),
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
A CD5+ B cell hybridoma derived factor(s), which induces maturation of CD5+, idiotype-specific B-cell populations.
January 1990, The Journal of molecular and cellular immunology : JMCI,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
A monoclonal anti-idiotype specific for human polyclonal IgM rheumatoid factor.
June 1992, Journal of immunology (Baltimore, Md. : 1950),
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
A high-affinity human monoclonal IgM antibody reacting with multiple strains of Mycoplasma hominis.
January 1990, Journal of clinical laboratory analysis,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Cryoprecipitation properties of a high-affinity monoclonal IgM anti-fluorescyl antibody.
December 1988, Molecular immunology,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Monoclonal IgM antibody exhibiting high-affinity binding and cryoglobulin properties.
August 1983, Proceedings of the National Academy of Sciences of the United States of America,
R W van der Heijden, and H Bunschoten, and A Hoek, and J Van Es, and M Punter, and A D Osterhaus, and F G Uytdehaag
Generation of a novel high-affinity monoclonal antibody with conformational recognition epitope on human IgM.
November 2010, Applied biochemistry and biotechnology,
Copied contents to your clipboard!