-transparent.png){kind=logo}
A bioluminescent rapid method (a three-hour test) has been developed to assess the susceptibility of microflora to antibiotics. It is based on comparison of intracellular ATP content in a sample (pus) after 3 h incubation at 37 degrees C in liquid culture medium (dilution: sample/culture medium--1:9) on a shaker in the presence and absence (control) of a single antibiotic concentration. A criterion has been offered for quantitative estimation of antibiotic susceptibility of microorganisms. The authors have optimized the measurements of intracellular ATP by the rapid bioluminescent method using immobilized firefly luciferase-based ATP reagent. The results of the method are in good correlation with those of the standard agar diffusion technique.
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
January 1964,
Voenno-meditsinskii zhurnal,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
January 1989,
Urologiia i nefrologiia,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
January 1964,
Laboratornoe delo,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
September 1951,
American journal of clinical pathology,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
August 1951,
The Journal of laboratory and clinical medicine,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
August 1961,
Canadian journal of microbiology,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
October 1990,
Vestnik khirurgii imeni I. I. Grekova,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
January 1986,
Laboratornoe delo,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
January 1997,
Prikladnaia biokhimiia i mikrobiologiia,
A I Titov, and
N A Romanova, and
T M Danilova, and
L Iu Brovko, and
N N Ugarova, and
P I Tolstykh
May 1992,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
