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The complementary adhesion molecules LFA-1 (CD11a, 18)/ICAM-1 (CD54) and LFA-2 (CD2)/LFA-3 (CD58) have been shown to be important in T cell interaction with lymphoid target cells. The role of these ligand pairs in cytotoxicity against somatic cells is less well established. While LFA-3 is expressed by all cells in the kidney, ICAM-1 expression is low in normal kidneys but is increased in allograft rejection. An in vitro cytotoxicity assay was used to examine the relative importance of the two adhesion ligands in immune damage against kidney cells in rejection. HLA-A2 specific cytotoxic T lymphocyte (CTL) recognition of cultured human kidney cells (HKC), of predominantly renal tubular cell origin, was studied. Immunofluorescence studies showed that both induced and uninduced HKC target cells expressed ICAM-1, MHC class I and LFA-3, but only MHC class I and class II antigens and ICAM-1 were significantly upregulated by cytokine induction. Effector cells expressed LFA-1 and LFA-2 but little or no ICAM-1 and LFA-3. Cytokine induction of ICAM-1 expression on HKC target cells increased their susceptibility to lysis. Monoclonal antibody against ICAM-1 or LFA-1 produced the greatest inhibition of HKC lysis, and their effects were not additive. Antibody against LFA-2 (CD2) or LFA-3 also produced significant inhibition, but to a lesser degree, and no additive effect was found.(ABSTRACT TRUNCATED AT 250 WORDS)
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
January 1989,
The Year in immunology,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
July 1998,
International journal of cancer,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
July 1990,
Journal of neuroimmunology,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
January 1989,
Virchows Archiv. B, Cell pathology including molecular pathology,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
October 1990,
European journal of immunology,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
December 1989,
Kidney international,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
July 1991,
Journal of immunology (Baltimore, Md. : 1950),
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
January 1981,
International journal of immunopharmacology,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
March 1978,
Cellular immunology,
M G Suranyi, and
G A Bishop, and
C Clayberger, and
A M Krensky, and
P Leenaerts, and
G Aversa, and
B M Hall
January 1988,
Annals of the New York Academy of Sciences,
