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We demonstrate here the usefulness of cytochalasin B enucleate cells for the study of the metabolism of cytoplasmic mRNA and for determining its half-life in animal cells. Simian virus 40 infected monkey cells in which the RNA had been labeled with [3H]uridine were enucleated, and the decay of the two prominent RNAs of simian virus 40, the 19S and 16S species, was measured by analysis on sucrose gradients. The results of these experiments, together with kinetic analysis of nuclear and cytoplasmic viral RNA, indicate a precursor-product relationship between the 19S and 16S cytoplasmic viral RNA species, which decay by first-order kinetics with a mean half-life of about 3 hr and 6 hr, respectively.
Y Aloni, and
M Shani, and
Y Reuveni
June 1974,
Journal of virology,
Y Aloni, and
M Shani, and
Y Reuveni
June 1972,
Proceedings of the National Academy of Sciences of the United States of America,
Y Aloni, and
M Shani, and
Y Reuveni
January 1972,
Journal of virology,
Y Aloni, and
M Shani, and
Y Reuveni
October 1980,
Chemico-biological interactions,
Y Aloni, and
M Shani, and
Y Reuveni
August 1980,
Proceedings of the National Academy of Sciences of the United States of America,
Y Aloni, and
M Shani, and
Y Reuveni
April 1972,
Proceedings of the National Academy of Sciences of the United States of America,
Y Aloni, and
M Shani, and
Y Reuveni
June 1971,
The Journal of general virology,
Y Aloni, and
M Shani, and
Y Reuveni
August 1973,
Journal of molecular biology,
Y Aloni, and
M Shani, and
Y Reuveni
January 1976,
Biochemical Society transactions,
Y Aloni, and
M Shani, and
Y Reuveni
March 1978,
Virology,
