-transparent.png){kind=logo}
The production of a factor with interleukin-3-like activity (IL-3-LA) by cultured human peripheral-blood mononuclear cells has been studied. Culture supernatants spontaneously derived from lymphocytes or monocytes stimulated the proliferative activity of 3 IL-3-dependent cell lines. Purification of this factor by gel filtration-high-pressure liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing demonstrated the presence of a glycoprotein with a molecular weight of 25-30 kilodaltons and an isoelectric point of 7.6. The biochemical characteristics of the IL-3-LA derived from human monocytes and lymphocytes were very similar. The biological activity of the semipurified factor was tested on mononuclear cells from fetal cord blood. It was found that after 21 days 40% of the cells in the culture were mature basophils which released 15-18 ng histamine/10(6) cells.
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
September 1985,
European journal of immunology,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
January 1989,
International journal of immunopharmacology,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
November 1990,
Clinical immunology and immunopathology,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
September 1996,
Journal of endocrinological investigation,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
August 1987,
Journal of immunology (Baltimore, Md. : 1950),
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
January 1993,
European cytokine network,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
May 1989,
The American journal of physiology,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
January 1992,
Pathobiology : journal of immunopathology, molecular and cellular biology,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
January 1991,
Journal of bioluminescence and chemiluminescence,
P Fishman, and
M Djaldetti, and
S Grossman, and
Y Sofer, and
B Sredni
October 1985,
Proceedings of the National Academy of Sciences of the United States of America,
