Differentiation of human embryonic stem cells into smooth muscle cells in adherent monolayer culture. 2006

Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
College of Life Sciences, Zhejiang University, No. 338, Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058, China.

Smooth muscle cell (SMC) plays critical roles in many human diseases, an in vitro system that recapitulates human SMC differentiation would be invaluable for exploring molecular mechanisms leading to the human diseases. We report a directed and highly efficient SMC differentiation system by treating the monolayer-cultivated human embryonic stem cells (hESCs) with all-trans retinoid acid (atRA). When the hESCs were cultivated in differentiation medium containing 10microM RA, more than 93% of the cells expressed SMC-marker genes along with the steadily accumulation of such SMC-specific proteins as SM alpha-actin and SM-MHC. The fully differentiated SMCs were stable in phenotype and capable of contraction. This inducible and highly efficient in vitro human SMC system could be an important resource to study the mechanisms of SMC phenotype determination in human.

UI MeSH Term Description Entries
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014212 Tretinoin An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE). Retinoic Acid,Vitamin A Acid,Retin-A,Tretinoin Potassium Salt,Tretinoin Sodium Salt,Tretinoin Zinc Salt,Vesanoid,all-trans-Retinoic Acid,beta-all-trans-Retinoic Acid,trans-Retinoic Acid,Acid, Retinoic,Acid, Vitamin A,Acid, all-trans-Retinoic,Acid, beta-all-trans-Retinoic,Acid, trans-Retinoic,Potassium Salt, Tretinoin,Retin A,Salt, Tretinoin Potassium,Salt, Tretinoin Sodium,Salt, Tretinoin Zinc,Sodium Salt, Tretinoin,Zinc Salt, Tretinoin,all trans Retinoic Acid,beta all trans Retinoic Acid,trans Retinoic Acid
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D053595 Embryonic Stem Cells Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells. Stem Cells, Embryonic,Cell, Embryonic Stem,Cells, Embryonic Stem,Embryonic Stem Cell,Stem Cell, Embryonic
D018929 Cell Culture Techniques Methods for maintaining or growing CELLS in vitro. Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell
D019070 Cell Lineage The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo. Cell Lineages,Lineage, Cell,Lineages, Cell
D032389 Myocytes, Smooth Muscle Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE). Smooth Muscle Cells,Cell, Smooth Muscle,Cells, Smooth Muscle,Myocyte, Smooth Muscle,Smooth Muscle Cell,Smooth Muscle Myocyte,Smooth Muscle Myocytes

Related Publications

Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
May 2008, Differentiation; research in biological diversity,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
January 2007, The International journal of developmental biology,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
March 2008, Current protocols in stem cell biology,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
July 2010, Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
May 2015, Journal of visualized experiments : JoVE,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
November 2012, Stem cell research,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
January 2011, Methods in molecular biology (Clifton, N.J.),
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
April 2009, American journal of physiology. Cell physiology,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
July 2017, European review for medical and pharmacological sciences,
Huarong Huang, and Xiaoli Zhao, and Liangbiao Chen, and Chao Xu, and Xing Yao, and Yongliang Lu, and Licheng Dai, and Ming Zhang
March 2010, Chinese medical journal,
Copied contents to your clipboard!