-transparent.png){kind=logo}
SV-40 DNA sheared form was coupled in a stable covalent bond to cyanogen bromide activated Sepharose. Under the conditions used at least 80% of the DNA was bound to Sepharose. The T 1/2 of hybridization of 0.5 mug/ml of SV-40 cRNA to SV-40 DNA-Sepharose was 1 hr. This rate of hybridization is sufficiently rapid to purify SV-40 sequences from solutions containing as little as 0.05-0.1 mug/ml. Nonspecific hybridization of RNA is in the range of 0.1-0.2% of the total input RNA. The DNA-Sepharose is fairly stable and can be reused several times to purify RNA. The SV-40 DNA-Sepharose was used to select large quantities of virus specific RNA from SV-40 infected BS-C-1 cells. The virus specific RNA when added to cell-free extracts from wheat germ was shown to direct the synthesis of the major viral structural protein VP-1.
E Gilboa, and
C L Prives, and
H Aviv
May 1976,
Biochemistry,
E Gilboa, and
C L Prives, and
H Aviv
February 1972,
FEBS letters,
E Gilboa, and
C L Prives, and
H Aviv
August 1973,
Proceedings of the National Academy of Sciences of the United States of America,
E Gilboa, and
C L Prives, and
H Aviv
August 1976,
Biotechnology and bioengineering,
E Gilboa, and
C L Prives, and
H Aviv
December 1978,
European journal of biochemistry,
E Gilboa, and
C L Prives, and
H Aviv
November 1969,
Biochimica et biophysica acta,
E Gilboa, and
C L Prives, and
H Aviv
January 1979,
Methods in enzymology,
E Gilboa, and
C L Prives, and
H Aviv
January 1972,
Archiv fur die gesamte Virusforschung,
E Gilboa, and
C L Prives, and
H Aviv
July 1972,
Molecular pharmacology,
E Gilboa, and
C L Prives, and
H Aviv
March 1973,
Biochimica et biophysica acta,
