-transparent.png){kind=logo}
1. The reaction of nitric oxide with oxidized and reduced ascorbate oxidase (L-ascorbate: oxygen oxidoreductase, EC 1.10.3.3) has been investigated by optical absorption measurements and electron paramagnetic resonance, and the results are compared with those of ceruloplasmin. 2. Upon anaerobic incubation of oxidized ascorbate oxidase with nitric oxide a decrease of the absorbance at 610 nm is found, which is due to an electron transfer from nitric oxide to Type-1 copper. 3. In the presence of nitric oxide the EPR absorbance of ascorbate oxidase decreases and shows predominatly a signal with characteristics of Type-2 copper (g parallel = 2.248; A parallel = 188 G), whereas the type-1 copper signal has vanished. 4. Comparison of the intensities of the EPR signals before and after NO-treatment points to the presence of one Type-2 and three Type-1 copper atoms per molecule of ascorbate oxidase. 5. It is shown that the changes in the optical and the EPR spectrum of ascorbate oxidase induced by nitric oxide are reversible. No difference in enzymic activity is found between the native enzyme and the NO-treated enzyme after removal of nitric oxide.
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
January 1996,
Methods in enzymology,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
October 2004,
FEBS letters,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
February 2006,
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
November 1977,
Biokhimiia (Moscow, Russia),
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
August 1996,
Biochemical Society transactions,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
July 2008,
Proceedings of the National Academy of Sciences of the United States of America,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
November 1998,
Circulation research,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
May 2006,
Nitric oxide : biology and chemistry,
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
June 2021,
Vision (Basel, Switzerland),
F X Leeuwen, and
R Wever, and
B F Gelder, and
L Avigliano, and
B Mondovi
July 2003,
The Journal of biological chemistry,
