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A murine anti-(human gastric carcinoma) monoclonal antibody, GL-013 (IgG1), which reacts with a high-molecular-mass glycoprotein from colorectal tumour tissue [Yang and Price (1989) Anticancer Res 9: 1707], was examined for reactivity against a panel of purified and partially purified antigens associated with tumours of the gastrointestinal tract. These included carcinoembryonic antigen (CEA), normal cross-reacting antigen, Y-hapten glycoproteins, and perchloric acid extracts and glycolipid preparations from colorectal tumours. While the GL-013 antibody failed to bind to these antigens, it was found to react strongly with synthetic peptides with sequences based upon that reported for the protein core of a human gastrointestinal mucin [Barnd et al. (1989) Proc Natl Acad Sci USA 86: 7159; Gum et al. (1989) J Biol Chem 264: 6480]. In control tests, a series of other anti-(colorectal tumour) antibodies (IgG1 and IgG3), with broad reactivity towards gastrointestinal carcinomas, as well as an anti-CEA antibody, (IgG1) failed to react with the synthetic peptides. It is concluded that the anti-(gastric carcinoma) monoclonal antibody GL-013 binds to a threonine-rich peptide epitope expressed within the protein core of gastrointestinal mucins.
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
January 1999,
Biospectroscopy,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
November 1984,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
November 1981,
The Journal of clinical investigation,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
December 1990,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
February 1996,
International journal of cancer,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
October 1998,
Disease markers,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
January 1988,
Dermatologica,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
October 1991,
Journal of cutaneous pathology,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
January 1998,
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine,
M R Price, and
M Sekowski, and
G Y Yang, and
L G Durrant, and
R A Robins, and
R W Baldwin
June 1990,
Gan no rinsho. Japan journal of cancer clinics,
