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Non-producer (NP) human cells induced by the Kirsten sarcoma virus were characterized. These morphologically altered NP cells produced neither infectious virus nor complement-fixing antigens of the murine sarcoma-leukemia virus complex. The NP cells did not release RNA-dependent DNA polymerase and type-C virus particles with a density of approximately 1.15 g/ml in sucrose gradients by 3H-uridine labelling. The NP cells produced tumors when transplanted subcutaneously into athymic nude mice. The tumor cells re-established in culture resembled the orginal NP cells, were confirmed as human cells by karyological analysis and were also found to be "non-producer". The sarcoma virus genome in NP cells could be rescued not only by co-cultivation with "helper virus"-releasing cells but also by superinfection with helper type-C viruses. Murine (Rauscher, Ki-MuLV, AT-124 and two other xenotropic viruses), feline, RD-114 and Simian (woolly monkey and baboon) type-C viruses possessed the ability to rescue the sarcoma genome from NP cells but not AKR leukemia virus. In addition, the feline leukemia virus titer obtained by the rescuing technique in NP cells was the same as those obtained in feline embryo and NP cells by CF induction assay.
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
November 1971,
The Journal of general virology,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
May 1979,
The Journal of general virology,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
February 1972,
Cancer research,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
August 1980,
The Journal of general virology,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
April 1985,
Cancer research,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
November 1981,
International journal of cancer,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
November 1977,
Journal of the National Cancer Institute,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
June 1979,
Virology,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
January 1982,
Journal of cellular physiology,
J S Rhim, and
H Y Cho, and
M L Vernon, and
P Arnstein, and
R J Huebner, and
R V Gilden
April 1973,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
