Sensitivity and specificity of an in-house rapid urease test for detecting Helicobacter pylori infection on gastric biopsy. 2006

Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. chariya@kku.ac.th

We developed an in-house rapid urease test (iRUT) and evaluated the efficacy and the agreement of the iRUT and the cRUT compared with culture and histology for the detection of H. pylori infection. Five iRUT media were tested with H. pylori isolates and other bacteria. The most suitable iRUT medium was further evaluated for detection of H. pylori infection. Gastric biopsies from 120 patients were diagnosed by culture, iRUT, cRUT and histology. The results of the iRUT and cRUT were read at 30 minutes, 1 hour and up to 24 hours. A true positive result was either the culture or both the RUT (cRUT or iRUT) and the histological examination being positive. The sensitivity and specificity of the iRUT result at 30 minutes, 1 hour and up to 24 hours were 77.1% and 100%, 77.6% and 100%, and 94.1% and 94.2%, respectively. Values for the same parameters of cRUT were 87.5% and 100%, 89.8% and 100%, and 100% and 94.2%, respectively. The agreement between the iRUT and cRUT was very good (kappa values > or = 0.82). Our results indicate that the iRUT is a-sensitive, specific and cost effective test. It can be appropriately applied for detecting H. pylori infection in gastric biopsy specimens.

UI MeSH Term Description Entries
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013270 Stomach An organ of digestion situated in the left upper quadrant of the abdomen between the termination of the ESOPHAGUS and the beginning of the DUODENUM. Stomachs
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014510 Urease An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5. Phytourease,Urea Amidohydrolase,Amidohydrolase, Urea
D015169 Colony Count, Microbial Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing. Agar Dilution Count,Colony-Forming Units Assay, Microbial,Fungal Count,Pour Plate Count,Spore Count,Spread Plate Count,Streak Plate Count,Colony Forming Units Assay, Microbial,Colony Forming Units Assays, Microbial,Agar Dilution Counts,Colony Counts, Microbial,Count, Agar Dilution,Count, Fungal,Count, Microbial Colony,Count, Pour Plate,Count, Spore,Count, Spread Plate,Count, Streak Plate,Counts, Agar Dilution,Counts, Fungal,Counts, Microbial Colony,Counts, Pour Plate,Counts, Spore,Counts, Spread Plate,Counts, Streak Plate,Dilution Count, Agar,Dilution Counts, Agar,Fungal Counts,Microbial Colony Count,Microbial Colony Counts,Pour Plate Counts,Spore Counts,Spread Plate Counts,Streak Plate Counts
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016480 Helicobacter pylori A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405). Campylobacter pylori,Campylobacter pylori subsp. pylori,Campylobacter pyloridis,Helicobacter nemestrinae

Related Publications

Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
June 2006, Nepal Medical College journal : NMCJ,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
August 2007, Journal of digestive diseases,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
July 1993, European journal of epidemiology,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
March 2012, Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
January 2002, Hepato-gastroenterology,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
October 2022, Iranian journal of microbiology,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
October 1998, Australian and New Zealand journal of medicine,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
January 2024, Diagnostic microbiology and infectious disease,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
January 1998, Acta gastro-enterologica Belgica,
Chariya Chomvarin, and Yingrit Chantarasuk, and Pisaln Mairiang, and Churairat Kularbkaew, and Apichat Sangchan, and Kitti Chanlertrith, and Wises Namwat
June 2002, Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
Copied contents to your clipboard!