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Leukocyte adherence to endothelium is in part mediated by the transient expression of endothelial-leukocyte adhesion molecule 1 (ELAM-1) on endothelial surfaces stimulated by tumor necrosis factor alpha (TNF), interleukin (IL) 1, or bacterial lipopolysaccharide (LPS). The intracellular factors controlling induction of ELAM-1 mRNA and protein are unknown. In nuclear runoff experiments with cultured human umbilical vein endothelial cells (HUVEC), we demonstrate that transcriptional activation of the ELAM-1 gene occurs following stimulation with TNF. Sequence analysis of the 5' flanking region of the ELAM-1 gene reveals consensus DNA-binding sequences for two known transcription factors, NF-kappa B and AP-1. Gel mobility shift assays demonstrate that TNF, IL-1, or LPS (but not IL-2, IL-4, IL-6, interferon gamma, histamine, or transforming growth factor beta) induces activation of NF-kappa B-like DNA binding activity in HUVEC. In contrast, neither TNF, IL-1, nor LPS activates proteins that bind to an AP-1 consensus sequence under these experimental conditions. Phorbol 12-myristate 13-acetate, a known activator of protein kinase C (PKC), weakly induces NF-kappa B-like activity, ELAM-1 mRNA, and ELAM-1 surface expression in HUVEC. However, TNF, IL-1, and LPS do not activate PKC in HUVEC at doses that strongly induce NF-kappa B-like protein activation and ELAM-1 gene expression. PKC blockade with H7 does not inhibit activation of these NF-kappa B-like proteins but does inhibit ELAM-1 gene transcription. We conclude that PKC-independent activation of NF-kappa B in HUVEC with TNF, IL-1, or LPS is associated with, but not sufficient for, activation of ELAM-1 gene transcription.
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
May 1994,
Neurology,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
February 1993,
Gan to kagaku ryoho. Cancer & chemotherapy,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
September 1992,
The Journal of biological chemistry,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
May 1991,
Nucleic acids research,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
May 1993,
Journal of periodontology,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
October 1991,
The Journal of clinical investigation,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
January 1997,
European urology,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
February 1991,
The British journal of dermatology,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
May 1992,
The Journal of clinical investigation,
K F Montgomery, and
L Osborn, and
C Hession, and
R Tizard, and
D Goff, and
C Vassallo, and
P I Tarr, and
K Bomsztyk, and
R Lobb, and
J M Harlan
June 2005,
Amyotrophic lateral sclerosis and other motor neuron disorders : official publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases,
