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Recognition sites of glycine tRNA for glycyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1. 2005

Koji Okamoto, and Atsushi Kuno, and Tsunemi Hasegawa
Graduate School of Science and Technology, Yamagata University, Yamagata 990-8560, Japan.

To elucidate the tRNA recognition sites of glycine tRNA from an extreme thermophilic and aerobic archaeon, Aeropyrum pernix K1, we examined glycylation activities using in vitro mutant glycine tRNA transcripts and recombinant A. pernix glycyl-tRNA synthetase. The recognition nucleotides were determined to be C35 and C36 of anticodon, C2-G71 and G3-C70 base-pairs of acceptor stem. However, discriminator base A73 was not recognized by glycyl-tRNA synthetase.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D006032 Glycine-tRNA Ligase An enzyme that activates glycine with its specific transfer RNA. EC 6.1.1.14. Glycyl T RNA Synthetase,Gly-tRNA Ligase,Glycyl-tRNA Synthetase,Gly tRNA Ligase,Glycine tRNA Ligase,Glycyl tRNA Synthetase,Ligase, Gly-tRNA,Ligase, Glycine-tRNA,Synthetase, Glycyl-tRNA
D012353 RNA, Transfer, Gly A transfer RNA which is specific for carrying glycine to sites on the ribosomes in preparation for protein synthesis. Glycine-Specific tRNA,Transfer RNA, Gly,tRNAGly,Iso tRNA(Gly),Iso-tRNA(Gly),tRNA(Gly),Gly Transfer RNA,Glycine Specific tRNA,RNA, Gly Transfer,tRNA, Glycine-Specific
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D041522 Aeropyrum A genus of anaerobic, chemolithotropic coccoid ARCHAEA, in the family DESULFUROCOCCACEAE. They live in marine environments.

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