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To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland epithelial cell strains from parotid or submandibular glands of human or macaque origin. Nineteen strains were derived from normal tissues and three from human parotid gland tumors. Both the normal and the tumor-derived salivary gland epithelial cells could be serially cultivated with the aid of a 3T3 fibroblast feeder layer in a mixture of Ham's F12 and Dulbecco's modified Eagle's media supplemented with fetal bovine serum, calcium, cholera toxin, hydrocortisone, insulin, and epidermal growth factor. Salivary gland epithelial cells cultured under these conditions conditioned to express the genes for at least two acinar-cell-specific markers at early passages. Amylase enzyme activity was detected in conditioned media from cultured rhesus parotid epithelial cells as late as Passage 5. Proline-rich-protein-specific RNAs were detected in primary cultures of both rhesus and human parotid epithelial cells. Neither amylase enzyme activity nor PRP-specific RNAs were detected in fibroblasts isolated from the same tissues. In addition, salivary gland epithelial cells cultured under our conditions retain the capacity to undergo dramatic morphologic changes in response to different substrata. The cultured salivary gland epithelial cells we have established will be important tools for the study of salivary gland differentiation and the tissue-specific regulation of salivary-type gene expression.
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
May 1978,
The Journal of investigative dermatology,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
December 1994,
In vitro cellular & developmental biology. Animal,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
June 1966,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
July 2019,
Journal of visualized experiments : JoVE,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
January 2013,
International journal of clinical and experimental pathology,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
June 1985,
The Journal of urology,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
June 2008,
Stem cells and development,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
October 1973,
The Journal of physiology,
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
September 2003,
Rheumatology (Oxford, England),
L M Sabatini, and
B L Allen-Hoffmann, and
T F Warner, and
E A Azen
January 1994,
Clinical and experimental rheumatology,
