Seasonal patterns of allozyme variation are examined for 12 polymorphic enzyme loci in Drosophila melanogaster. The data derive from a total of 56 samples taken from a natural population in the Summer and Fall of 1978 and 1979. Samples were obtained at approximately five-day intervals and assayed for 6-phosphogluconate dehydrogenase (6Pgd), phosphoglucomutase (Pgm) and glucose-6-phosphate dehydrogenase (G6pd). The remaining nine enzymes were assayed in an average of eight samples per season. None of the loci exhibit regular seasonal cycles of gene-frequency change, although 6Pgd does show significant, but irregular, frequency oscillations. There is also little evidence for gene-frequency differences between years, although 6Pgd is again exceptional in showing significant frequency changes between years. In addition, genotypic frequency distributions are usually consistent with random mating expectations. With the notable exception of 6Pgd, the data give a strong impression of gene-frequency homogeneity within and among years, despite obvious seasonal changes in climate and in the distribution of breeding sites.