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Temperature-induced stress on growth and expression of hsp in freshwater alga Scenedesmus quadricauda. 2006

S Zargar, and K Krishnamurthi, and S Saravana Devi, and T K Ghosh, and T Chakrabarti
Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur-440 020 (MS), India. santoshzargar@yahoo.com

OBJECTIVE To investigate the impact of various levels of sublethal temperature (26 degrees C, 31 degrees C, 33 degrees C, 36 degrees C, and 39 degrees C) on growth and heat shock protein (hsp) expression in freshwater green alga Scenedesmus quadricauda. METHODS Impact of selected levels of temperature on growth rate (based on optical density), population count, chlorophyll-a and biomass of the alga was evaluated in artificial growth medium for 19 days. To determine the induction of hsp in the alga, it was exposed to selected temperature levels for 3 h and further kept for 6 h at culturing condition at 26 degrees C. Induction of hsp was confirmed by immuno-detection followed by SDS-polyacrylamide gel electrophoresis. RESULTS The selected growth parameters such as growth rate, population count, chlorophyll-a and biomass were reduced significantly (P < 0.001) at 39 degrees C. However, hsp 70 expression was observed only at 39 degrees C. CONCLUSIONS Temperature up to 36 degrees C may be considered as the limit of safe exposure for thermal stress for the alga Scenedesmus quadricauda.

UI MeSH Term Description Entries
D005618 Fresh Water Water containing no significant amounts of salts, such as water from RIVERS and LAKES. Freshwater,Fresh Waters,Freshwaters,Water, Fresh,Waters, Fresh
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D044444 Scenedesmus A genus of GREEN ALGAE in the family Scenedesmaceae. It forms colonies of usually four or eight cylindrical cells that are widely distributed in freshwater and SOIL.
D018533 Biomass Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop. Biomasses
D018840 HSP70 Heat-Shock Proteins A class of MOLECULAR CHAPERONES found in both prokaryotes and in several compartments of eukaryotic cells. These proteins can interact with polypeptides during a variety of assembly processes in such a way as to prevent the formation of nonfunctional structures. Heat-Shock Proteins 70,Heat Shock 70 kDa Protein,Heat-Shock Protein 70,HSP70 Heat Shock Proteins,Heat Shock Protein 70,Heat Shock Proteins 70,Heat-Shock Proteins, HSP70
D020418 Algal Proteins Proteins found in any species of algae. Algal Gene Product,Algal Gene Products,Algal Gene Protein,Algal Gene Proteins,Algal Peptide,Algal Peptides,Algal Protein,Gene Products, Algal,Gene Product, Algal,Gene Protein, Algal,Gene Proteins, Algal,Peptide, Algal,Peptides, Algal,Product, Algal Gene,Protein, Algal,Protein, Algal Gene,Proteins, Algal

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