Immunovisualization of high (HK) and low (LK) molecular weight kininogens on isolated human neutrophils. 1992

C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
Institute of Histology and Pathology, Austral University, Valdivia, Chile.

An immunocytochemical study was performed to examine the cellular localization and the subcellular distribution of kininogens in human blood cells. Kininogens were visualised using the immunogold-silver staining method and confocal scanning laser microscopy. We confirmed the existence of high molecular weight kininogen in human neutrophils and describe for the first time the presence of low molecular weight kininogen on these cells. Both high and low molecular weight kininogens were restricted to the neutrophils where they localized as clusters of immunogold particles on the cell membrane. No labeling was observed intracellularly in organelles such as mitochondria, endoplasmic reticulum, and azurophilic or specific granules after permeabilization of the neutrophils with Triton X-100, a procedure that permitted the visualization of elastase in the azurophilic granules. Clusters of high molecular weight kininogen molecules attached to the neutrophil surface could serve as receptors for plasma kallikrein and/or be the source of substrate for a discrete and circumscribed formation of kinins that may in turn facilitate the local diapedesis of neutrophils and the transudation of plasma constituents during acute inflammation.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007704 Kininogens Endogenous peptides present in most body fluids. Certain enzymes convert them to active KININS which are involved in inflammation, blood clotting, complement reactions, etc. Kininogens belong to the cystatin superfamily. They are cysteine proteinase inhibitors. HIGH-MOLECULAR-WEIGHT KININOGEN; (HMWK); is split by plasma kallikrein to produce BRADYKININ. LOW-MOLECULAR-WEIGHT KININOGEN; (LMWK); is split by tissue kallikrein to produce KALLIDIN. Cystatins, Kininogen,Kininogen,Prekinins,Prokinins,T-Kininogen,Thiostatin,Kininogen Cystatins,T Kininogen
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
April 2004, Journal of leukocyte biology,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
March 2000, Thrombosis research,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
October 1974, Journal of biochemistry,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
December 1972, Biochemical and biophysical research communications,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
July 1994, Thrombosis and haemostasis,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
November 1987, Biochemistry,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
January 1989, Advances in experimental medicine and biology,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
January 1979, Thrombosis research,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
February 1984, British journal of haematology,
C D Figueroa, and L M Henderson, and J Kaufmann, and R A De La Cadena, and R W Colman, and W Müller-Esterl, and K D Bhoola
January 1983, Nature,
Copied contents to your clipboard!