[Activation of p38 mitogen-activated protein kinase pathway in ventilator-induced lung injury in rat]. 2007

Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.

OBJECTIVE To study the activation of p38 mitogen-activated protein kinase (MAPK) and the expression of inflammatory cytokines in ventilator-induced lung injury (VILI) in rat. METHODS Thirty healthy male SD rats were randomly divided into three group (A, B, C group, n=10 per group). Mechanical ventilation was instituted in all the groups. A group: tidal volume (V(T))=8 ml/kg, respiratory rate (RR)=80/min; B group: V(T)=20 ml/kg, RR=80/min; C group: V(T)=40 ml/kg, RR=80/min. The time of ventilation for all the groups was two hours. Rats were sacrificed after experiment was finished. The bronchial lavage liquid and lung tissue were collected and stored with routine methods. The pathological changes in lung tissue were examined with optical microscope. The expression of p38 and phos-p38 (p-p38) were measured by Western blotting in lung tissues. The expression of intercellular adhesion molecular-1 (ICAM-1) was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The contents of total protein, white blood cells (WBC), myeloperoxidase (MPO), macrophage inflammatory protein-2 (MIP-2) and tumor necrosis factor-alpha (TNF-alpha) were also determined. RESULTS Compared with A group, total protein, WBC, MPO, MIP-2, TNF-alpha, ICAM-1 and p-p38 were significantly increased in B group and C group (all P<0.01). Compared with B group, the above indexes were also significantly increased in C group (P<0.05 or P<0.01). CONCLUSIONS Large V(T) mechanical ventilation can significantly activate the p-p38 and inflammatory cytokines, which may play an important role in VILI.

UI MeSH Term Description Entries
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008297 Male Males
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D012121 Respiration, Artificial Any method of artificial breathing that employs mechanical or non-mechanical means to force the air into and out of the lungs. Artificial respiration or ventilation is used in individuals who have stopped breathing or have RESPIRATORY INSUFFICIENCY to increase their intake of oxygen (O2) and excretion of carbon dioxide (CO2). Ventilation, Mechanical,Mechanical Ventilation,Artificial Respiration,Artificial Respirations,Mechanical Ventilations,Respirations, Artificial,Ventilations, Mechanical
D001992 Bronchoalveolar Lavage Fluid Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung. Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats

Related Publications

Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
January 2012, Journal of leukocyte biology,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
February 2005, Chinese medical journal,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
October 2008, Journal of endodontics,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
May 2009, Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
December 2014, Human immunology,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
July 2010, American journal of respiratory cell and molecular biology,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
January 2001, Transplantation proceedings,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
February 2008, PloS one,
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
June 2011, Inflammation research : official journal of the European Histamine Research Society ... [et al.],
Dan Feng, and Shang-long Yao, and You Shang, and Qing-ping Wu, and Li-kui Wang
February 2008, Critical care medicine,
Copied contents to your clipboard!