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An enzymatic method for cholesterol in serum [Clin. Chem. 20, 470 (1974)] was initially found to be unsatisfactory for measuring cholesterol in high-density-lipoprotein fractions prepared by precipitation with Mn2+. A fine precipitate formed in the cuvette and cholesterol values were falsely increased. We describe a simple, convenient method for circumventing these problems. An ethylenediaminetetraacetate solution is used to reconstitute the enzymatic reagent. Cholesterol values by this procedure correlated with those obtained by the Lipid Research Clinic's procedure for the same lipoprotein fraction preparations (regression slope, .998; Y-intercept, 8.9 mg/liter; correlation coefficient, .984; standard error of the estimate, 16.8 mg/liter). Precision of the assay, including the precipitation step, was calculated. The SDwithin day was 9.7 mg/liter and SDoverall was 23.7 mg/liter. Results for total cholesterol with the modified reagent were linearly related to concentrations exceeding 4 g/liter, thereby permitting determination of high-density-lipoproteins and total cholesterol in a single run.
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
May 1976,
Clinical chemistry,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
June 1979,
Rinsho byori. The Japanese journal of clinical pathology,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
October 1986,
Clinical chemistry,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
January 1978,
Clinical chemistry,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
December 1986,
Scandinavian journal of clinical and laboratory investigation,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
August 1980,
Clinical chemistry,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
November 1981,
The American journal of cardiology,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
February 1985,
Clinical chemistry,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
March 1994,
Clinical chemistry,
B W Steele, and
D F Koehler, and
M M Azar, and
T P Blaszkowski, and
K Kuba, and
M E Dempsey
May 1977,
Clinical chemistry,
