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Protein phosphokinase activity from the calf ovary cytosol (105000 X g supernatant fraction) has been resolved by chromatography and polyacrylamide gel electrophoresis into two major protein kinases, PK-H1 and PK-H2, both dependent on adenosine 3':5'-monophosphate (cyclic AMP). The enzymes have similar molecular weights (230000) and substrate specificities but differ in their cyclic-AMP-dependency and stimulation by cyclic AMP. The differences have been explained by the presence in PK-H1 of a unique cyclic-AMP-binding protein which has little catalytic activity associated with it. The cyclic-AMP-binding protein has a high affinity for cyclic AMP and in addition is able to inhibit the activity of the isolated catalytic subunit. The ovarian cyclic-AMP-dependent protein kinases have properties similar to those found in other tissues. They can be dissociated into catalytic and regulatory subunits and are inhibited by a heat-stable protein inhibitor isolated from rabbit skeletal muscle. Preincubation of the cytosol with high levels of cyclic AMP resulted in additional cyclic-AMP-dependent protein kinases and cyclic-AMP-binding proteins which include protein kinases and binding proteins of greater than 400 000 molecular weight.
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
August 1977,
Proceedings of the National Academy of Sciences of the United States of America,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
November 1974,
Biochimica et biophysica acta,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
January 1979,
Journal of cyclic nucleotide research,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
September 1970,
Biochemical and biophysical research communications,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
April 1980,
The Journal of biological chemistry,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
August 1977,
Biochimica et biophysica acta,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
December 1971,
Biochemical and biophysical research communications,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
February 1975,
The Journal of biological chemistry,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
September 1978,
Revista espanola de fisiologia,
D W Talmadge, and
E Bechtel, and
A Salonkangas, and
P Huber, and
R A Jungmann, and
U Eppenberger
January 1976,
The Journal of investigative dermatology,
