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The TATA box-binding factor TFIID plays a primary role in the process of transcription initiation by RNA polymerase II and its regulation by various gene-specific factors. Here we employ a permuted binding site/gel retardation assay with recombinant yeast and human TFIID to show that this factor induces DNA bending around the TATA element. These results are consistent with the presence of G + C-rich sequence elements flanking the consensus TATA element and led to the recently confirmed suggestion that TFIID interacts with the TATA element via the minor groove. They also raise the possibility that TFIID-induced bending might facilitate promoter interactions of other general factors in the preinitiation complex or interactions between general transcription factors and regulatory factors bound at upstream sites.
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
May 1993,
Biochemical and biophysical research communications,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
September 1993,
Proceedings of the National Academy of Sciences of the United States of America,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
January 1994,
Molecular and cellular biology,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
July 1992,
Proceedings of the National Academy of Sciences of the United States of America,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
July 1998,
Molecular and cellular biology,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
July 1993,
FEBS letters,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
May 1992,
Molecular and cellular biology,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
March 1993,
Nucleic acids research,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
April 1992,
Proceedings of the National Academy of Sciences of the United States of America,
M Horikoshi, and
C Bertuccioli, and
R Takada, and
J Wang, and
T Yamamoto, and
R G Roeder
December 1995,
Proceedings of the National Academy of Sciences of the United States of America,
