Clinical comparison of grafts stored in McCarey-Kaufman medium at 4 degrees C and in corneal organ culture at 31 degrees C. 1992

W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
Cornea Service, Rotterdam, The Netherlands.

Twenty-eight paired human corneas were preserved in minimal essential medium at 31 degrees C and in McCarey-Kaufman medium at 4 degrees C. These grafts were then transplanted in pairs of patients with keratoconus who were age matched as closely as possible. These pairs received donor corneas from the same donor, so for each pair the donor age and time from death to preservation were the same. Visual acuity, central corneal thickness, and endothelial cell counts were compared. During the 1- to 2-year study period, no statistically significant difference in visual acuity, corneal thickness, or endothelial cell density was found between grafts stored in minimal essential medium and those stored in McCarey-Kaufman medium.

UI MeSH Term Description Entries
D007640 Keratoconus A noninflammatory, usually bilateral protrusion and thinning of the CORNEA, the apex being displaced downward and nasally. It occurs most commonly in females at about puberty. Two closely related noninflammatory corneal ectasias are pellucid marginal degeneration and keratoglobus. Keratoglobus,Pellucid Marginal Corneal Degeneration,Pellucid Marginal Degeneration,Degeneration, Pellucid Marginal,Marginal Degeneration, Pellucid,Pellucid Marginal Degenerations
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009924 Organ Culture Techniques A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1) Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D009926 Organ Preservation The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Organ Preservations,Preservation, Organ,Preservations, Organ
D009930 Organic Chemicals A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form. Organic Chemical,Chemical, Organic,Chemicals, Organic
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D002452 Cell Count The number of CELLS of a specific kind, usually measured per unit volume or area of sample. Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D003315 Cornea The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed) Corneas
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004728 Endothelium, Corneal Single layer of large flattened cells covering the surface of the cornea. Anterior Chamber Epithelium,Corneal Endothelium,Endothelium, Anterior Chamber,Epithelium, Anterior Chamber,Anterior Chamber Endothelium

Related Publications

W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
January 1998, Eye (London, England),
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
February 1984, Archives of ophthalmology (Chicago, Ill. : 1960),
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
November 1987, American journal of ophthalmology,
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
March 1982, Ophthalmic surgery,
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
August 2022, Indian journal of ophthalmology,
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
February 1986, Archives of ophthalmology (Chicago, Ill. : 1960),
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
April 1978, Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology,
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
January 1981, Ophthalmology,
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
January 1985, Developments in ophthalmology,
W J Rijneveld, and W H Beekhuis, and G van Rij, and B Rinkel-van Driel, and E Pels
January 1971, Acta chirurgiae plasticae,
Copied contents to your clipboard!