Comparison of fast liquid chromatography/tandem mass spectrometric methods for simultaneous determination of cladribine and clofarabine in mouse plasma. 2007

Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
Drug Metabolism and Pharmacokinetics Department, Schering Plough Research Institute, Kenilworth, NJ 07033, USA. yunsheng.hsieh@spcorp.com

Several fast high performance liquid chromatography/atmospheric pressure ionization/tandem mass spectrometric (HPLC-API/MS/MS) methods were evaluated for the simultaneous determination of cladribine and clofarabine in mouse plasma samples. The chemical separation for analytes under reversed-phase conditions were achieved by using either ultra-performance liquid chromatography (UPLC) or micro-column HPLC coupled to either a quadrupole linear ion trap mass spectrometer (QTrap MS) or a triple quadrupole mass spectrometer. Atmospheric pressure chemical ionization (APCI) or atmospheric pressure photoionization (APPI) interfaces in the positive mode were employed prior to mass spectrometric detection. The effects of various dopant solvents on the APPI sensitivities of analytes and the internal standard were investigated. The matrix ionization suppression potential for the test compounds in plasma samples on fast HPLC-MS/MS methods was examined by a post-column infusion technique. In this work, these proposed approaches were successfully employed to determine the concentrations of cladribine and clofarabine in mouse plasma in the low ng/ml region. The mouse plasma levels of all analytes obtained by these fast HPLC-MS/MS methods were compared and found to be well correlated in terms of analytical accuracy.

UI MeSH Term Description Entries
D007166 Immunosuppressive Agents Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging. Immunosuppressant,Immunosuppressive Agent,Immunosuppressants,Agent, Immunosuppressive,Agents, Immunosuppressive
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D007654 Ketoconazole Broad spectrum antifungal agent used for long periods at high doses, especially in immunosuppressed patients. Nizoral,R-41400,R41,400,R41400,R 41400
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D000077866 Clofarabine An adenine arabinonucleoside derivative that acts as an antineoplastic antimetabolite. It is used in the treatment of ACUTE LYMPHOID LEUKEMIA in pediatric patients who have relapsed. 9H-Purin-6-amine, 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-,2-Chloro-2'-arabino-fluoro-2'-deoxyadenosine,2-Chloro-2'-fluoroarabino-2'-deoxyadenosine,2-Chloro-9-(2-deoxy-2-fluoro-beta-D-arbinofuranosyl)adenine,2-Chloro-9-(2-deoxy-2-fluoroarabinofuranosyl)adenine,Cl-F-ara-A,Clofarex,Clolar,Evoltra,2 Chloro 2' arabino fluoro 2' deoxyadenosine,2 Chloro 2' fluoroarabino 2' deoxyadenosine
D000227 Adenine Nucleotides Adenine Nucleotide,Adenosine Phosphate,Adenosine Phosphates,Nucleotide, Adenine,Nucleotides, Adenine,Phosphate, Adenosine,Phosphates, Adenosine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000935 Antifungal Agents Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues. Anti-Fungal Agents,Antifungal Agent,Fungicides, Therapeutic,Antibiotics, Antifungal,Therapeutic Fungicides,Agent, Antifungal,Anti Fungal Agents,Antifungal Antibiotics
D001087 Arabinonucleosides Nucleosides containing arabinose as their sugar moiety. Arabinofuranosylnucleosides

Related Publications

Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
January 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
February 2011, Journal of mass spectrometry : JMS,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
March 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
March 2016, Zhonghua yi xue za zhi,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
July 2010, Journal of separation science,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
December 2014, Biomedical chromatography : BMC,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
September 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
July 2007, Archives of pharmacal research,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
June 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Yunsheng Hsieh, and Christine J G Duncan, and Suining Lee, and Ming Liu
September 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Copied contents to your clipboard!