BIOMAT Database Logo BIOMAT Database Logo

Control of sigma virus multiplication by the ref(2)P gene of Drosophila melanogaster: an in vivo study of the PB1 domain of Ref(2)P. 2007

A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Université Versailles SQY, CNRS, Laboratoire de Génétique et Biologie Cellulaire-UMR 8159, 78035 Versailles, France.

Ref(2)P has been described as one of the Drosophila proteins that interacts with the sigma virus cycle. We generated alleles to identify critical residues involved in the restrictive (inhibiting viral multiplication) or permissive (allowing viral multiplication) character of Ref(2)P. We demonstrate that permissive alleles increase the ability of the sigma virus to infect Drosophila when compared to null alleles and we confirm that restrictive alleles decrease this capacity. Moreover, we have created alleles unfunctional in viral cycling while functional for Ref(2)P fly functions. This type of allele had never been observed before and shows that fly- and virus-related activities of Ref(2)P are separable. The viral status of Ref(2)P variants is determined by the amino-terminal PB1 domain polymorphism. In addition, an isolated PB1 domain mimics virus-related functions even if it is similar to a loss of function toward fly-related activities. The evolutionary tree of the Ref(2)P PB1 domain that we could build on the basis of the natural allele sequences is in agreement with an evolution of PB1 domain due to successive transient selection waves.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D004331 Drosophila melanogaster A species of fruit fly frequently used in genetics because of the large size of its chromosomes. D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D000483 Alleles Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product. Allelomorphs,Allele,Allelomorph
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012209 Rhabdoviridae A family of bullet-shaped viruses of the order MONONEGAVIRALES, infecting vertebrates, arthropods, protozoa, and plants. Genera include VESICULOVIRUS; LYSSAVIRUS; EPHEMEROVIRUS; NOVIRHABDOVIRUS; Cytorhabdovirus; and Nucleorhabdovirus. Rhabdoviruses,Varicosavirus,Rhabdovirus,Varicosaviruses
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications

Related Publications

A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Unusual variability of the Drosophila melanogaster ref(2)P protein which controls the multiplication of sigma rhabdovirus.
April 1993, Genetics,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Localization of domains within the Drosophila Ref(2)P protein involved in the intracellular control of sigma rhabdovirus multiplication.
July 1995, Journal of virology,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
[Locus ref(2)P of Drosophila melanogaster. I. Cytogenetic localization of ref(2)P].
January 1986, Biology of the cell,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Molecular analysis of ref(2)P, a Drosophila gene implicated in sigma rhabdovirus multiplication and necessary for male fertility.
November 1989, The EMBO journal,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Immunological cross-reactions and interactions between the Drosophila melanogaster ref(2)P protein and sigma rhabdovirus proteins.
June 1993, Journal of virology,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
[Dosage effects of the non permissive allele of Drosophila ref(2)P gene on sensitive strains of sigma virus (author's transl)].
February 1978, Molecular & general genetics : MGG,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Effect of actinomycin D and mitomycin C on sigma virus multiplication in Drosophila melanogaster.
January 1973, Virology,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Study of the ref(2)P locus of Drosophila melanogaster. II. Genetic studies of the 37DF region.
June 1993, Molecular & general genetics : MGG,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
The sigma virus infection of Drosophila melanogaster.
January 1968, Current topics in microbiology and immunology,
A Carré-Mlouka, and S Gaumer, and P Gay, and A M Petitjean, and C Coulondre, and P Dru, and F Bras, and S Dezélée, and D Contamine
Genetic and molecular features of Su(P), a gene that interacts with ref(2)P in male fertility of Drosophila melanogaster.
April 2001, Molecular genetics and genomics : MGG,
Copied contents to your clipboard!