BIOMAT Database Logo BIOMAT Database Logo

PCR amplification of long DNA fragments. 1992

M R Ponce, and J L Micol
Division of Biology, California Institute of Technology, Pasadena 91125.

UI MeSH Term Description Entries
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016296 Mutagenesis Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS. Mutageneses

Related Publications

M R Ponce, and J L Micol
[An approach for PCR amplification of long DNA fragments].
October 1994, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
M R Ponce, and J L Micol
Uniform terminus PCR: amplification of minute unknown DNA fragments.
November 1998, BioTechniques,
M R Ponce, and J L Micol
PCR amplification and silver stain detection of genomic DNA fragments.
September 1989, Trends in genetics : TIG,
M R Ponce, and J L Micol
Adaptor PCR for the specific amplification of unknown DNA fragments.
January 1998, BioTechniques,
M R Ponce, and J L Micol
Long-PCR amplification of human genomic DNA.
January 2011, Methods in molecular biology (Clifton, N.J.),
M R Ponce, and J L Micol
Use of buccal epithelial cells for PCR amplification of large DNA fragments.
June 2000, The Journal of forensic odonto-stomatology,
M R Ponce, and J L Micol
[A method of selective PCR-amplification of genomic DNA fragments (SAGF method)].
September 1995, Biokhimiia (Moscow, Russia),
M R Ponce, and J L Micol
Rapid generation of DNA fragments by PCR amplification of crude, synthetic oligonucleotides.
May 1990, Nucleic acids research,
M R Ponce, and J L Micol
Long PCR amplification of large fragments of viral genomes: a technical overview.
January 2003, Methods in molecular biology (Clifton, N.J.),
M R Ponce, and J L Micol
XL PCR amplification of long targets from genomic DNA.
January 2002, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!