Density and diameter of dentinal tubules in etched and non-etched bovine dentine examined by scanning electron microscopy. 2007

Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
Department of Operative Dentistry, University of Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida e Souza 200, Mogi das Cruzes, SP, Brazil.

Bovine teeth have been widely used in studies focusing adhesion to dentine over the last years. However, little is known about main structural aspects of bovine dentine, especially regarding density and diameter of its tubules. Thirty bovine incisors were randomly divided into two groups. In group I, teeth were cross-sectioned at three depths: outer, middle and inner. The dentinal surfaces were etched with 35% phosphoric acid for 90s. In group II, the teeth were fractured at the same three depths. All the specimens were processed and examined in a Jeol 6100 SEM. Fifteen micrographs from each depth were obtained for the two groups at a magnification of 2500x. To determine the diameter of the dentinal tubules, the LEICA Q500 MC software was used. All the results were statistically processed in the EXCEL software. The dentinal tubules were wider at the outer regions (GI: 5.21+/-0.64microm; GII: 2.30+/-0.09microm) than that at the inner regions (GI: 2.71+/-0.72microm; GII: 1.77+/-0.06microm). Tubular density was higher at the inner regions (50310+/-11178tubules/mm(2)) than that at the outer regions (18772+/-2951tubules/mm(2)). In addition, the peritubular dentine was wider at the outer depth than at the inner one. Our results showed that bovine dentine possesses some structural aspects different from those previously reported for human dentine, mainly related to the diameter of dentinal tubules and to the thickness of peritubular dentine at the several depths.

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D008855 Microscopy, Electron, Scanning Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY. Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D010756 Phosphoric Acids Inorganic derivatives of phosphoric acid (H3PO4). Note that organic derivatives of phosphoric acids are listed under ORGANOPHOSPHATES. Pyrophosphoric Acids,Acids, Phosphoric,Acids, Pyrophosphoric
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003804 Dentin The hard portion of the tooth surrounding the pulp, covered by enamel on the crown and cementum on the root, which is harder and denser than bone but softer than enamel, and is thus readily abraded when left unprotected. (From Jablonski, Dictionary of Dentistry, 1992) Dentine,Dentines,Dentins
D000134 Acid Etching, Dental Preparation of TOOTH surfaces and DENTAL MATERIALS with etching agents, usually phosphoric acid, to roughen the surface to increase adhesion or osteointegration. Dental Acid Etching,Etching, Dental Acid
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013314 Stress, Mechanical A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area. Mechanical Stress,Mechanical Stresses,Stresses, Mechanical
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
May 2000, Archives of oral biology,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
January 2002, The Journal of clinical pediatric dentistry,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
November 2000, SADJ : journal of the South African Dental Association = tydskrif van die Suid-Afrikaanse Tandheelkundige Vereniging,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
June 1994, Scandinavian journal of dental research,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
January 1984, Archives of oral biology,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
January 1985, Archives of oral biology,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
September 2003, Minerva stomatologica,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
December 1972, The Journal of Nihon University School of Dentistry,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
January 1976, Archives of oral biology,
Maristela Dutra-Correa, and Camillo Anauate-Netto, and Victor E Arana-Chavez
February 1985, The Anatomical record,
Copied contents to your clipboard!