Alpha 2,6-sialyltransferase I expression in the placenta of patients with preeclampsia. 2007

Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, National Yang-Ming University School of Medicine, Taipei, Taiwan, R.O.C. phwang@vghtpe.gov.tw

BACKGROUND The expression of sialyl-glycoconjugates changes during development, differentiation and oncogenic transformation, tumor invasion and metastasis. Similarly, in the early stage of pregnancy, trophoblast cells have to undergo adhesion, invasion, and proliferation to develop a healthy placenta; the cytobiologic behavior is similar to tumor growth and invasion. Inadequate trophoblast invasion to the spiral artery in the 2nd trimester of pregnancy was believed to be correlated with pregnancy complications, including preeclampsia. METHODS Alterations in alpha2,6-sialyltransferase I (ST6Gal I) mRNA in the placental tissues of women with preeclampsia (n=20) and without preeclampsia (n=20 used as a control) were examined by semiquantitative reverse transcription-polymerase chain reaction and real-time quantitative reverse transcription-polymerase chain reaction. The transcription regulators of ST6Gal I including a "constitutive" promoter (Y + Z form), "hepatic" promoter (H form), and lymphoblastic promoter (X form) were investigated. The enzyme activity of ST6Gal I was also examined. RESULTS Both mRNA expression and enzyme activity of ST6Gal I did not show a significant difference in the placental tissues of the women of both groups. The transcription regulators of ST6Gal I, including the Y+Z form and the H form, also failed to show any difference. The X form, seldom detected in the study, was excluded from analysis. CONCLUSIONS Our results suggested that ST6Gal I was not involved in the pathogenesis of the preeclampsia.

UI MeSH Term Description Entries
D010920 Placenta A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES). Placentoma, Normal,Placentome,Placentas,Placentomes
D011225 Pre-Eclampsia A complication of PREGNANCY, characterized by a complex of symptoms including maternal HYPERTENSION and PROTEINURIA with or without pathological EDEMA. Symptoms may range between mild and severe. Pre-eclampsia usually occurs after the 20th week of gestation, but may develop before this time in the presence of trophoblastic disease. Toxemias, Pregnancy,EPH Complex,EPH Gestosis,EPH Toxemias,Edema-Proteinuria-Hypertension Gestosis,Gestosis, EPH,Hypertension-Edema-Proteinuria Gestosis,Preeclampsia,Preeclampsia Eclampsia 1,Pregnancy Toxemias,Proteinuria-Edema-Hypertension Gestosis,Toxemia Of Pregnancy,1, Preeclampsia Eclampsia,1s, Preeclampsia Eclampsia,EPH Toxemia,Eclampsia 1, Preeclampsia,Eclampsia 1s, Preeclampsia,Edema Proteinuria Hypertension Gestosis,Gestosis, Edema-Proteinuria-Hypertension,Gestosis, Hypertension-Edema-Proteinuria,Gestosis, Proteinuria-Edema-Hypertension,Hypertension Edema Proteinuria Gestosis,Of Pregnancies, Toxemia,Of Pregnancy, Toxemia,Pre Eclampsia,Preeclampsia Eclampsia 1s,Pregnancies, Toxemia Of,Pregnancy Toxemia,Pregnancy, Toxemia Of,Proteinuria Edema Hypertension Gestosis,Toxemia Of Pregnancies,Toxemia, EPH,Toxemia, Pregnancy,Toxemias, EPH
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D012799 Sialyltransferases A group of enzymes with the general activity CMP-N-acetylneuraminate:acceptor N-acetylneuraminyl transferase. They catalyze the transfer of N-ACETYLNEURAMINIC ACID from CMP-N-ACETYLNEURAMINIC ACID to an acceptor, which is usually the terminal sugar residue of an oligosaccharide, a glycoprotein, or a glycolipid. Glycoprotein Sialyltransferases,Glycosyltransferase Family 29,Sialyltransferase,Ectosialyltransferase,Glycoprotein Sialyltransferase,Sialyltransferase, Glycoprotein,Sialyltransferases, Glycoprotein
D015703 Antigens, CD Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation. CD Antigen,Cluster of Differentiation Antigen,Cluster of Differentiation Marker,Differentiation Antigens, Leukocyte, Human,Leukocyte Differentiation Antigens, Human,Cluster of Differentiation Antigens,Cluster of Differentiation Markers,Antigen Cluster, Differentiation,Antigen, CD,CD Antigens,Differentiation Antigen Cluster,Differentiation Marker Cluster,Marker Cluster, Differentiation
D020133 Reverse Transcriptase Polymerase Chain Reaction A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols. Polymerase Chain Reaction, Reverse Transcriptase,Reverse Transcriptase PCR,PCR, Reverse Transcriptase,Transcriptase PCR, Reverse

Related Publications

Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
January 1994, The Journal of biological chemistry,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
January 1996, Acta neuropathologica,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
July 2000, Journal of biotechnology,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
January 1989, The Journal of biological chemistry,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
May 1995, Biochemical and biophysical research communications,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
December 1995, Biology of reproduction,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
September 1995, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
July 1994, The Journal of biological chemistry,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
January 1998, Langenbecks Archiv fur Chirurgie. Supplement. Kongressband. Deutsche Gesellschaft fur Chirurgie. Kongress,
Peng-Hui Wang, and Wen-Ling Lee, and Yu-Hui Yang, and Yi-Jen Chen, and Ying-Chieh Tsai, and Chiou-Chung Yuan
May 1992, The Journal of biological chemistry,
Copied contents to your clipboard!