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Effects of additives on the thermostability of chloroperoxidase. 2007

Lifei Zhi, and Yucheng Jiang, and Yingsong Wang, and Mancheng Hu, and Shuni Li, and Yingjun Ma
School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.

The effects of several polyhydroxy compounds (glucose, fructose, gumsugar, galactose, trehalose, dextran, xylose, PEG200, glycerin) and surfactant (dioctyl sulfosuccinate sodium salt, AOT) on the catalytic activity and thermal stability of chloroperoxidase (CPO) in aqueous systems were investigated at various temperatures. A 25% superactivity was found in AOT solutions at 25 degrees C, and it could be maintained during the 882 h. PEG200 and glycerin were proven to be the most efficient stabilizer for CPO in temperatures ranging from 25 to 60 degrees C. Trehalose is more helpful than other sugars for extended storage of CPO. These results are promising in view of industrial applications of this versatile biological catalyst. The protective mechanism of various additives on CPO was discussed.

UI MeSH Term Description Entries
D007211 Indoles Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D009930 Organic Chemicals A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form. Organic Chemical,Chemical, Organic,Chemicals, Organic
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D002711 Chloride Peroxidase An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. EC 1.11.1.10. Chloroperoxidase,Peroxidase, Chloride
D003911 Dextrans A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes. Dextran,Dextran 40,Dextran 40000,Dextran 70,Dextran 75,Dextran 80,Dextran B-1355,Dextran B-1355-S,Dextran B1355,Dextran B512,Dextran Derivatives,Dextran M 70,Dextran T 70,Dextran T-40,Dextran T-500,Hemodex,Hyskon,Infukoll,Macrodex,Polyglucin,Promit,Rheodextran,Rheoisodex,Rheomacrodex,Rheopolyglucin,Rondex,Saviosol,Dextran B 1355,Dextran B 1355 S,Dextran T 40,Dextran T 500
D004143 Dioctyl Sulfosuccinic Acid All-purpose surfactant, wetting agent, and solubilizer used in the drug, cosmetics, and food industries. It has also been used in laxatives and as cerumenolytics. It is usually administered as either the calcium, potassium, or sodium salt. Dioctyl Sulfosuccinates,Docusate,Aerosol OT,Colace,DEH-Na-SS,DOSS,Diethylhexyl Sodium Sulfosuccinate,Dioctyl Sulfosuccinate,Dioctyl Sulfosuccinic Acid, Ammonium Salt,Dioctyl Sulfosuccinic Acid, Barium Salt,Dioctyl Sulfosuccinic Acid, Calcium Salt,Dioctyl Sulfosuccinic Acid, Magnesium Salt,Dioctyl Sulfosuccinic Acid, Potassium Salt,Dioctyl Sulfosuccinic Acid, Sodium Salt,Dioctylsulfosuccinate,Docusate Calcium,Docusate Potassium,Docusate Sodium,Sodium Bis(2-ethylhexyl)sulfosuccinate,Sodium Dioctyl Sulfosuccinate,Sodium Dioctylsulphosuccinate,Sulfosuccinic Acid bis(2-Ethylhexyl) Ester,DEH Na SS,Dioctyl Sulfosuccinate, Sodium,Dioctylsulphosuccinate, Sodium,Sodium Sulfosuccinate, Diethylhexyl,Sulfosuccinate, Diethylhexyl Sodium,Sulfosuccinate, Dioctyl,Sulfosuccinates, Dioctyl
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004795 Enzyme Stability The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat. Enzyme Stabilities,Stabilities, Enzyme,Stability, Enzyme
D005632 Fructose A monosaccharide in sweet fruits and honey that is soluble in water, alcohol, or ether. It is used as a preservative and an intravenous infusion in parenteral feeding. Levulose,Apir Levulosa,Fleboplast Levulosa,Levulosa,Levulosa Baxter,Levulosa Braun,Levulosa Grifols,Levulosa Ibys,Levulosa Ife,Levulosa Mein,Levulosado Bieffe Medit,Levulosado Braun,Levulosado Vitulia,Plast Apyr Levulosa Mein,Levulosa, Apir,Levulosa, Fleboplast

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