BIOMAT Database Logo BIOMAT Database Logo

Identification of three gene regions associated with virulence in Dichelobacter nodosus, the causative agent of ovine footrot. 1991

M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Department of Microbiology, Monash University, Clayton, Victoria, Australia.

Dichelobacter nodosus (formerly Bacteroides nodosus) is a Gram-negative strict anaerobe and is the primary pathogen involved in ovine footrot. A comparative hybridization strategy was used to isolate recombinant clones which hybridized to DNA from a virulent strain of D. nodosus but not with a benign isolate. Three virulence-associated gene regions were identified and one of these regions was shown to be present in multiple copies in the D. nodosus genome. Hybridization studies on 101 clinical isolates of D. nodosus showed that these strains could be divided into three hybridization categories which could be correlated with the virulence of the isolates. The recombinant clones have considerable potential for the development of a gene-probe-based method for the differential diagnosis of ovine footrot.

UI MeSH Term Description Entries
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001439 Bacteroides A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D012757 Sheep Diseases Diseases of domestic and mountain sheep of the genus Ovis. Ovine Diseases,Disease, Ovine,Disease, Sheep,Diseases, Ovine,Diseases, Sheep,Ovine Disease,Sheep Disease
D014774 Virulence The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS. Pathogenicity
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot

Related Publications

M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Virulence regions and virulence factors of the ovine footrot pathogen, Dichelobacter nodosus.
December 1996, FEMS microbiology letters,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
The intD mobile genetic element from Dichelobacter nodosus, the causative agent of ovine footrot, is associated with the benign phenotype.
October 2009, Anaerobe,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
The site-specific integration of genetic elements may modulate thermostable protease production, a virulence factor in Dichelobacter nodosus, the causative agent of ovine footrot.
October 1999, Microbiology (Reading, England),
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
A polymerase chain reaction assay for improved determination of virulence of Dichelobacter nodosus, the specific causative pathogen for ovine footrot.
February 1995, Veterinary microbiology,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Electroporation-mediated transformation of the ovine footrot pathogen Dichelobacter nodosus.
December 1998, FEMS microbiology letters,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Identification and characterization of Dichelobacter nodosus serogroup H from ovine footrot in India.
August 2016, Anaerobe,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Assessment of a rtPCR for the detection of virulent and benign Dichelobacter nodosus, the causative agent of ovine footrot, in Australia.
August 2018, BMC veterinary research,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Survival of the ovine footrot pathogen Dichelobacter nodosus in different soils.
April 2016, Anaerobe,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Amino acid sequence of extracellular acidic protease V5 of Dichelobacter nodosus, the causative organism of ovine footrot.
April 1993, Biochemistry and molecular biology international,
M E Katz, and P M Howarth, and W K Yong, and G G Riffkin, and L J Depiazzi, and J I Rood
Prevalence of Dichelobacter nodosus and Ovine Footrot in German Sheep Flocks.
April 2021, Animals : an open access journal from MDPI,
Copied contents to your clipboard!