Effect of hepatocyte growth factor on long term hematopoiesis of human progenitor cells in transgenic-severe combined immunodeficiency mice. 2007

Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai City, Osaka, Japan. sugiura@vet.osakafu-u.ac.jp

Hepatocyte growth factor (HGF), which was originally isolated as a liver generating factor, enhances hematopoiesis. To study the effect of HGF on hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs), we generated severe combined immunodeficiency (SCID) mice producing human (h) HGF and/or stem cell factor (SCF) by transferring the relevant genes to fertilized eggs, and then transplanted hematopoietic progenitors from human cord blood into the transgenic (Tg) SCID mice. Six months after transplantation, a significantly larger number of human cells were found in the Tg SCID mice than in non-Tg controls. Characteristically, the recipient SCID mice producing h HGF (HGF-SCID) had a significantly increased number of h CD41+ cells, whereas the SCF-SCID recipients had more CD11b+ cells. Significantly large numbers of CD34+ progenitors were found in the SCID mice transferred with both h HGF and h SCF genes (HGF/SCF-SCID) when compared with HGF-SCID or SCF-SCID mice. These results imply that HGF supports the differentiation of progenitors in megakaryocyte lineage, whereas SCF supports that in myeloid lineage. The results also imply that HGF acts on HSCs/HPCs as a synergistic proliferative factor combined with SCF. We have demonstrated the advantage of the human cytokine-producing animal in the maintenance of human HSCs.

UI MeSH Term Description Entries
D008297 Male Males
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006410 Hematopoiesis The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY). Hematopoiesis, Medullary,Haematopoiesis,Medullary Hematopoiesis
D006412 Hematopoietic Stem Cells Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood. Colony-Forming Units, Hematopoietic,Progenitor Cells, Hematopoietic,Stem Cells, Hematopoietic,Hematopoietic Progenitor Cells,Cell, Hematopoietic Progenitor,Cell, Hematopoietic Stem,Cells, Hematopoietic Progenitor,Cells, Hematopoietic Stem,Colony Forming Units, Hematopoietic,Colony-Forming Unit, Hematopoietic,Hematopoietic Colony-Forming Unit,Hematopoietic Colony-Forming Units,Hematopoietic Progenitor Cell,Hematopoietic Stem Cell,Progenitor Cell, Hematopoietic,Stem Cell, Hematopoietic,Unit, Hematopoietic Colony-Forming,Units, Hematopoietic Colony-Forming
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016513 Mice, SCID Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice. SCID Mice,SCID-hu Mice,Severe Combined Immunodeficient Mice,Immunodeficient Mice, Severe Combined,Mouse, SCID,Mouse, SCID-hu,Mice, SCID-hu,Mouse, SCID hu,SCID Mouse,SCID hu Mice,SCID-hu Mouse

Related Publications

Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
September 1997, Blood,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
October 1998, International journal of experimental pathology,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
January 1985, Journal of clinical immunology,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
February 1993, Transplantation proceedings,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
March 2007, Laboratory investigation; a journal of technical methods and pathology,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
February 1994, Cancer research,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
January 1996, Research communications in molecular pathology and pharmacology,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
April 1994, Hepatology (Baltimore, Md.),
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
July 1993, Biochemical and biophysical research communications,
Kikuya Sugiura, and Shigeru Taketani, and Tomoo Yoshimura, and Tomoyoshi Nishino, and Naoki Nishino, and Jun-ichi Fujisawa, and Hiroko Hisha, and Toshio Inaba, and Susumu Ikehara
January 1978, Bulletin du cancer,
Copied contents to your clipboard!