Antigenicity of infectious pancreatic necrosis virus VP2 subviral particles expressed in yeast. 2007

F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
Advanced BioNutrition Inc., 7155-H Columbia Gateway Drive, Columbia, MD 21046, USA.

Infectious pancreatic necrosis (IPN) virus, the etiologic agent of infectious pancreatic necrosis in salmonid fish, causes significant losses to the aquaculture industry. The gene for the viral capsid protein (VP2) was cloned into a yeast expression vector and expressed in Saccharomyces cerevisae. Expression of the capsid gene in yeast resulted in formation of approximately 20nm subviral particles composed solely of VP2 protein. Anti-IPNV antibodies were detected in rainbow trout vaccinated either by injection of purified VP2-subviral particles (rVP2-SVP) or by feeding recombinant yeast expressing rVP2-SVP. Challenge of rVP2-SVP immunized trout with a heterologous IPNV strain and subsequent viral load determination demonstrated that both injection and orally vaccinated fish had lower IPNV loads than naive or sham-vaccinated fish. This study demonstrates the ability of rVP2-SVPs to induce a specific immune response and the ability of immunized fish to reduce the viral load after an experimentally induced IPNV infection.

UI MeSH Term Description Entries
D007114 Immunization Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow). Immunologic Stimulation,Immunostimulation,Sensitization, Immunologic,Variolation,Immunologic Sensitization,Immunological Stimulation,Sensitization, Immunological,Stimulation, Immunologic,Immunizations,Immunological Sensitization,Immunological Sensitizations,Immunological Stimulations,Sensitizations, Immunological,Stimulation, Immunological,Stimulations, Immunological,Variolations
D007274 Injections, Intraperitoneal Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall. Intraperitoneal Injections,Injection, Intraperitoneal,Intraperitoneal Injection
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005393 Fish Diseases Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates). Disease, Fish,Diseases, Fish,Fish Disease
D005814 Genes, Viral The functional hereditary units of VIRUSES. Viral Genes,Gene, Viral,Viral Gene
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated

Related Publications

F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
July 2018, Fish & shellfish immunology,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
December 1990, Vaccine,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
April 2001, Fish & shellfish immunology,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
November 2015, Fish & shellfish immunology,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
April 2009, Journal of virological methods,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
December 2007, Viral immunology,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
March 1995, The Journal of general virology,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
March 2014, Journal of aquatic animal health,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
January 1978, Archives of virology,
F C Thomas Allnutt, and Robert M Bowers, and Christopher G Rowe, and Vikram N Vakharia, and Scott E LaPatra, and Arun K Dhar
August 2021, Fish & shellfish immunology,
Copied contents to your clipboard!