Detection of Escherichia coli O157: H7 vt and rfb(O157) by multiplex polymerase chain reaction. 2007

Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfb(O157) encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfb(O157), respectively. Multiplex PCR detected reference strain O157: H7 (NF-7777) with a sensitivity of 10(5) CFU per ml with no amplification of other 15 pathogenic bacteria. After incubation of 10(2) CFU/25 gram raw meat in tryptic soy broth at 37 degrees C for 8 hours, multiplex PCR conducted with the addition of 100 mg bovine serum albumin produced the two specific PCR products for E. coli O157: H7. This modified multiplex PCR is a rapid, sensitive, and specific technique for detecting and differentiating E. coli O157: H7 and has the potential to be used as an alternative to conventional methods for the screening of O157: H7 strains isolated from raw meat.

UI MeSH Term Description Entries
D008460 Meat The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game. Meats
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001427 Bacterial Toxins Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases. Bacterial Toxin,Toxins, Bacterial,Toxin, Bacterial
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D013785 Thailand Formerly known as Siam, this is a Southeast Asian nation at the center of the Indochina peninsula. Bangkok is the capital city. Kingdom of Thailand,Siam
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide
D019453 Escherichia coli O157 A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin. E coli O157,E coli O157-H7,Escherichia coli O157-H7

Related Publications

Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
January 1998, Microbiology and immunology,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
January 2002, Journal of food protection,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
September 1996, International journal of food microbiology,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
January 2003, Journal of food protection,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
September 2004, Medical and veterinary entomology,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
July 2000, Journal of food protection,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
August 1995, Journal of clinical microbiology,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
February 2006, Journal of microbiology (Seoul, Korea),
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
August 2000, Journal of food protection,
Apirak Visetsripong, and Kobchai Pattaragulwanit, and Jiraporn Thaniyavarn, and Ryosuke Matsuura, and Akio Kuroda, and Orasa Sutheinkul
July 2022, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases,
Copied contents to your clipboard!