-transparent.png){kind=logo}
Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions. 2007
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan. akao_t@nrib.go.jp
We performed random sequencing of cDNAs from nine biologically or industrially important cultures of the industrially valuable fungus Aspergillus oryzae to obtain expressed sequence tags (ESTs). Consequently, 21 446 raw ESTs were accumulated and subsequently assembled to 7589 non-redundant consensus sequences (contigs). Among all contigs, 5491 (72.4%) were derived from only a particular culture. These included 4735 (62.4%) singletons, i.e. lone ESTs overlapping with no others. These data showed that consideration of culture grown under various conditions as cDNA sources enabled efficient collection of ESTs. BLAST searches against the public databases showed that 2953 (38.9%) of the EST contigs showed significant similarities to deposited sequences with known functions, 793 (10.5%) were similar to hypothetical proteins, and the remaining 3843 (50.6%) showed no significant similarity to sequences in the databases. Culture-specific contigs were extracted on the basis of the EST frequency normalized by the total number for each culture condition. In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories.
Related Publications
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
March 2009,
Letters in applied microbiology,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
January 2006,
Fungal genetics and biology : FG & B,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
October 2005,
Fungal genetics and biology : FG & B,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
June 2011,
DNA research : an international journal for rapid publication of reports on genes and genomes,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
June 1994,
Plant molecular biology,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
July 1994,
Molecular and biochemical parasitology,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
May 2008,
Plant cell reports,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
October 2005,
Functional & integrative genomics,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
April 2013,
Molecular biology reports,
Takeshi Akao, and
Motoaki Sano, and
Osamu Yamada, and
Terumi Akeno, and
Kaoru Fujii, and
Kuniyasu Goto, and
Sumiko Ohashi-Kunihiro, and
Kumiko Takase, and
Makoto Yasukawa-Watanabe, and
Kanako Yamaguchi, and
Yoko Kurihara, and
Jun-ichi Maruyama, and
Praveen Rao Juvvadi, and
Akimitsu Tanaka, and
Yoji Hata, and
Yasuji Koyama, and
Shotaro Yamaguchi, and
Noriyuki Kitamoto, and
Katsuya Gomi, and
Keietsu Abe, and
Michio Takeuchi, and
Tetsuo Kobayashi, and
Hiroyuki Horiuchi, and
Katsuhiko Kitamoto, and
Yutaka Kashiwagi, and
Masayuki Machida, and
Osamu Akita
August 2009,
Journal of microbiology and biotechnology,
