Insights into rubber biosynthesis from transcriptome analysis of Hevea brasiliensis latex. 2007

Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
Biotechnology Unit, Rubber Research Institute of Malaysia, Malaysian Rubber Board, RRIM Experiment Station, 47000 Sungai Buloh, Selangor, Malaysia. kschow@lgm.gov.my

Hevea brasiliensis is the most widely cultivated species for commercial production of natural rubber (cis-polyisoprene). In this study, 10,040 expressed sequence tags (ESTs) were generated from the latex of the rubber tree, which represents the cytoplasmic content of a single cell type, in order to analyse the latex transcription profile with emphasis on rubber biosynthesis-related genes. A total of 3,441 unique transcripts (UTs) were obtained after quality editing and assembly of EST sequences. Functional classification of UTs according to the Gene Ontology convention showed that 73.8% were related to genes of unknown function. Among highly expressed ESTs, a significant proportion encoded proteins related to rubber biosynthesis and stress or defence responses. Sequences encoding rubber particle membrane proteins (RPMPs) belonging to three protein families accounted for 12% of the ESTs. Characterization of these ESTs revealed nine RPMP variants (7.9-27 kDa) including the 14 kDa REF (rubber elongation factor) and 22 kDa SRPP (small rubber particle protein). The expression of multiple RPMP isoforms in latex was shown using antibodies against REF and SRPP. Both EST and quantitative reverse transcription-PCR (QRT-PCR) analyses demonstrated REF and SRPP to be the most abundant transcripts in latex. Besides rubber biosynthesis, comparative sequence analysis showed that the RPMPs are highly similar to sequences in the plant kingdom having stress-related functions. Implications of the RPMP function in cis-polyisoprene biosynthesis in the context of transcript abundance and differential gene expression are discussed.

UI MeSH Term Description Entries
D007840 Latex A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS. Latices,Latice
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D012408 Rubber A high-molecular-weight polymeric elastomer derived from the milk juice (LATEX) of HEVEA brasiliensis and other trees and plants. It is a substance that can be stretched at room temperature to at least twice its original length and after releasing the stress, retract rapidly, and recover its original dimensions fully. Latex Rubber,Elastica,India Rubber,Natural Rubber,Plant Rubber,Vulcanite,Natural Rubbers,Plant Rubbers,Rubber, Natural,Rubber, Plant,Rubbers, Natural,Rubbers, Plant
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D016415 Sequence Alignment The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms. Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA

Related Publications

Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
November 1957, Plant physiology,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
December 1963, The Biochemical journal,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
May 2018, Physiology and molecular biology of plants : an international journal of functional plant biology,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
September 1969, The Biochemical journal,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
July 2021, Materials science & engineering. C, Materials for biological applications,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
March 1976, Canadian journal of microbiology,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
January 1991, Planta,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
January 2016, Journal of proteomics,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
July 1995, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
Keng-See Chow, and Kiew-Lian Wan, and Mohd Noor Mat Isa, and Azlina Bahari, and Siang-Hee Tan, and K Harikrishna, and Hoong-Yeet Yeang
November 1989, The Journal of biological chemistry,
Copied contents to your clipboard!