Biomaterial Database

[Detection of gingipian gene of Porphyromonas gingivalis in subgingival plaque]. 2007

Chen Li, and Yu-qin Gao, and Ya-ping Pan, and Li Lin, and Ming Zhong

Department of Periodontology, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China.

OBJECTIVE The purpose of this study was to detect two genes (kgp-cd and rgpB-cd) of Porphyromonas gingivalis in subgingival plaque, and determine the relationship between the two genes and the periodontal clinical indexes. METHODS Eighty four subgingival plaque samples were included in this study. The extracted DNA was amplified with the primers designed to obtain the genes encoding the catalytic domain of kgp and rgpB (kgp-cd, rgpB-cd), and typing P.gingivalis into two genotypes. The relationship between different genotypes of P. gingivalis and the periodontal clinical indexes was statistically analyzed by SPSS11.5 software package for Student's t test and Chi-square test. RESULTS The detection rate of type-A P.gingivalis (85.29%) was higher than that of type-B P.gingivalis (14.71%), P<0.05. Both probe depth and bleeding on probing had significant difference between different genotypes of P.gingivalis (PD:t=2.85,P<0.05;BOP:P<0.05). CONCLUSIONS kgp-cd and rgpB-cd were related to pathogenicity of P.gingivalis.

UI	MeSH Term	Description	Entries
D003546	Cysteine Endopeptidases	ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
D003773	Dental Plaque	A film that attaches to teeth, often causing DENTAL CARIES and GINGIVITIS. It is composed of MUCINS, secreted from salivary glands, and microorganisms.	Plaque, Dental
D005798	Genes, Bacterial	The functional hereditary units of BACTERIA.	Bacterial Gene,Bacterial Genes,Gene, Bacterial
D005838	Genotype	The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.	Genogroup,Genogroups,Genotypes
D005884	Gingival Hemorrhage	The flowing of blood from the marginal gingival area, particularly the sulcus, seen in such conditions as GINGIVITIS, marginal PERIODONTITIS, injury, and ASCORBIC ACID DEFICIENCY.	Hemorrhage, Gingival,Gingival Hemorrhages
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016966	Porphyromonas gingivalis	A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium produces a cell-bound, oxygen-sensitive collagenase and is isolated from the human mouth.	Bacteroides gingivalis
D018829	Adhesins, Bacterial	Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.	Adhesins, Fimbrial,Bacterial Adhesins,Fimbrial Adhesins,Adhesin, Bacterial,Bacterial Adhesin