Intraspecific variation of second internal transcribed spacer of nuclear ribosomal DNA among populations of Anopheles (Kerteszia) cruzii (Diptera: Culicidae). 2007

Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000, São Paulo, SP, Brazil. rmalafronte@usp.br

Anopheline species of the subgenus Kerteszia, including Anopheles (Kerteszia) cruzii Dyar & Knab (Diptera: Culicidae), are bromeliad-malaria vectors in the Atlantic rain forest of Brazil. Morphological, genetic, and molecular polymorphisms among different populations of An. cruzii have been reported, and it has been suggested that this taxon includes a complex of cryptic species. Specimens of An. cruzii were collected in the states of SHo Paulo and Santa Catarina, from locations where autochthonous malaria cases have been reported during the last decade. The second internal transcribed spacers (ITS2) of nuclear ribosomal DNA of the captured specimens were sequenced and compared with each other. Intraspecific ITS2 sequence polymorphisms were identified, and nucleotide divergence among specimens varied from 0.3 to 0.9%. The number of nucleotides in the ITS2 sequences of these mosquitoes varied from 327 to 334, and the CG contents varied from 61.7 to 62%. The data provide further indication of An. cruzii being a complex of cryptic species.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000852 Anopheles A genus of mosquitoes (CULICIDAE) that are known vectors of MALARIA. Anopheles gambiae
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D015252 Deoxyribonucleases, Type II Site-Specific Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4. DNA Restriction Enzymes, Type II,DNase, Site-Specific, Type II,Restriction Endonucleases, Type II,Type II Restriction Enzymes,DNase, Site Specific, Type II,Deoxyribonucleases, Type II, Site Specific,Deoxyribonucleases, Type II, Site-Specific,Site-Specific DNase, Type II,Type II Site Specific DNase,Type II Site Specific Deoxyribonucleases,Type II Site-Specific DNase,Type II Site-Specific Deoxyribonucleases,Deoxyribonucleases, Type II Site Specific,Site Specific DNase, Type II
D016415 Sequence Alignment The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms. Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations
D021903 DNA, Ribosomal Spacer The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers). Ribosomal Spacer DNA,Spacer DNA, Ribosomal

Related Publications

Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
August 2004, Memorias do Instituto Oswaldo Cruz,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
May 1994, Molecular biology and evolution,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
October 1958, Revista brasileira de malariologia e doencas tropicais. Publicacoes avulsas,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
December 1993, International journal for parasitology,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
May 2008, Journal of medical entomology,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
July 2005, Journal of medical entomology,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
January 2002, Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
April 2010, Acta tropica,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
September 2005, Journal of medical entomology,
Rosely Dos S Malafronte, and Mauro T Marrelli, and Corália C L Ramirez, and Maíra N Nassar, and Osvaldo Marinotti
December 2003, Memorias do Instituto Oswaldo Cruz,
Copied contents to your clipboard!