BIOMAT Database Logo BIOMAT Database Logo

Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans. 2007

Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Department of Physics, National Tsing-Hua University, Hsinchu 30013, Taiwan.

The branched-chain amino-acid aminotransferase (BCAT), which requires pyridoxal 5'-phosphate (PLP) as a cofactor, is a key enzyme in the biosynthetic pathway of the hydrophobic amino acids leucine, isoleucine and valine. DrBCAT from Deinococcus radiodurans, which has a molecular weight of 40.9 kDa, was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data to 2.50 A resolution from a DrBCAT crystal, the crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 56.37, b = 90.70, c = 155.47 A. Preliminary analysis indicates the presence of two DrBCAT molecules in the asymmetric unit, with a solvent content of 47.52%.

UI MeSH Term Description Entries
D007532 Isoleucine An essential branched-chain aliphatic amino acid found in many proteins. It is an isomer of LEUCINE. It is important in hemoglobin synthesis and regulation of blood sugar and energy levels. Alloisoleucine,Isoleucine, L-Isomer,L-Isoleucine,Isoleucine, L Isomer,L-Isomer Isoleucine
D007930 Leucine An essential branched-chain amino acid important for hemoglobin formation. L-Leucine,Leucine, L-Isomer,L-Isomer Leucine,Leucine, L Isomer
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D000637 Transaminases A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1. Aminotransferase,Aminotransferases,Transaminase
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D014633 Valine A branched-chain essential amino acid that has stimulant activity. It promotes muscle growth and tissue repair. It is a precursor in the penicillin biosynthetic pathway. L-Valine,L Valine
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies
D034301 Deinococcus A genus of gram-positive aerobic cocci found in the soil, that is highly resistant to radiation, especially ionizing radiation (RADIATION, IONIZING). Deinococcus radiodurans is the type species. Deinococcus radiodurans

Related Publications

Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Crystallization and preliminary X-ray crystallographic analysis of a putative agmatinase from Deinococcus radiodurans.
October 2004, Acta crystallographica. Section D, Biological crystallography,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Expression, purification, crystallization and preliminary X-ray crystallographic studies of Deinococcus radiodurans thioredoxin reductase.
August 2006, Acta crystallographica. Section F, Structural biology and crystallization communications,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Purification, crystallization and preliminary X-ray analysis of two nudix hydrolases from Deinococcus radiodurans.
April 2003, Acta crystallographica. Section D, Biological crystallography,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Expression, purification and preliminary X-ray crystallographic analysis of UDP-galactopyranose mutase from Deinococcus radiodurans.
August 2009, Acta crystallographica. Section F, Structural biology and crystallization communications,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Expression, purification, crystallization and preliminary X-ray diffraction analysis of acylpeptide hydrolase from Deinococcus radiodurans.
September 2014, Acta crystallographica. Section F, Structural biology communications,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Purification, crystallization and preliminary X-ray diffraction analysis of DNA damage response A protein from Deinococcus radiodurans.
December 2010, Acta crystallographica. Section F, Structural biology and crystallization communications,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Purification, crystallization and preliminary crystallographic analysis of DR0248, an MNT-HEPN fused protein from Deinococcus radiodurans.
January 2015, Acta crystallographica. Section F, Structural biology communications,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Purification, crystallization and preliminary crystallographic investigation of FrnE, a disulfide oxidoreductase from Deinococcus radiodurans.
November 2014, Acta crystallographica. Section F, Structural biology communications,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Crystallization and preliminary X-ray characterization of branched-chain amino acid aminotransferase from Escherichia coli.
May 1989, Journal of biochemistry,
Chung-Der Chen, and Tien-Feng Huang, and Chih-Hao Lin, and Hong-Hsiang Guan, and Yin-Cheng Hsieh, and Yi-Hung Lin, and Yen-Chieh Huang, and Ming-Yih Liu, and Wen-Chang Chang, and Chun-Jung Chen
Crystallization and preliminary X-ray crystallographic analysis of the RecR protein from Deinococcus radiodurans, a member of the RecFOR DNA-repair pathway.
February 2004, Acta crystallographica. Section D, Biological crystallography,
Copied contents to your clipboard!