Determination of Sudan dye residues in eggs by liquid chromatography and gas chromatography-mass spectrometry. 2007

Limin He, and Yijuan Su, and Binghu Fang, and Xiangguang Shen, and Zhenling Zeng, and Yahong Liu
Department of Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong PC 510642, PR China.

A sensitive and cheap high performance liquid chromatography (HPLC) with ultraviolet-visible (UV-VIS) was developed for the determination of Sudan dyes (I, II, III, and IV) residues in various types of eggs. The chromatographic separation was achieved on a reverse phase C18 column with gradient elution, using a mobile phase of 0.1% formic acid acetonitrile/0.1% formic acid aqueous solution; detector was set at 478 nm for Sudan I and 520 nm for Sudan II, III and IV. The suspected egg samples were derivatized with N,O-bis (trimethylsilyl) trifluoro-acetamide and confirmed by gas chromatography-mass spectrometry (GC-MS) in EI. Mass spectra of trimethylsilyl derivatives of the Sudan dyes were built up in EI mode. Recoveries of the Sudan dyes ranged between 79.8 and 95.7% in eggs by HPLC-UV, with all the relative standard deviations of less than 5%. Limit of detection (LOD), limit of quantification (LOQ) were in the range of 4.0-4.8 and 12.3-13.8 microg kg(-1) in eggs, respectively. Identification and confirmation could be validated in the range of 2.0-4.2 microg kg(-1) with the GC-MS method. This method is suitable for routine fast monitoring, screening and confirmation of Sudan dyes residues in eggs, as mandated by regulatory agencies.

UI MeSH Term Description Entries
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D004396 Coloring Agents Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS. Coloring Agent,Dye,Dyes,Organic Pigment,Stain,Stains,Tissue Stain,Tissue Stains,Organic Pigments,Pigments, Inorganic,Agent, Coloring,Inorganic Pigments,Pigment, Organic,Pigments, Organic,Stain, Tissue,Stains, Tissue
D004531 Eggs Animal reproductive bodies, or the contents thereof, used as food. The concept is differentiated from OVUM, the anatomic or physiologic entity.
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry

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