Plasmodium vivax thrombospondin related adhesion protein: immunogenicity and protective efficacy in rodents and Aotus monkeys. 2007

Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
Instituto de Inmunología del Valle, Universidad del Valle, Cali, Colombia.

The thrombospondin related adhesion protein (TRAP) is a malaria pre-erythrocytic antigen currently pursued as malaria vaccine candidate to Plasmodium falciparum. In this study, a long synthetic peptide (LSP) representing a P. vivax TRAP fragment involved in hepatocyte invasion was formulated in both Freund and Montanide ISA 720 adjutants and administered by IM and subcutaneous routes to BALB/c mice and Aotus monkeys. We measured specific humoral immune responses in both animal species and performed a sporozoite challenge in Aotus monkeys to assess the protective efficacy of the vaccine. After immunization both mice and Aotus seroconverted as shown by ELISA, and the specific anti-peptide antibodies cross reacted with the parasite in IFAT assays. Only two out of six immunized animals became infected after P. vivax sporozoite challenge as compared with four out of six animals from the control group. These results suggest that this TRAP fragment has protective potential against P. vivax malaria and deserves further studies as vaccine candidate.

UI MeSH Term Description Entries
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010865 Pilot Projects Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work. Pilot Studies,Pilot Study,Pilot Project,Project, Pilot,Projects, Pilot,Studies, Pilot,Study, Pilot
D010966 Plasmodium vivax A protozoan parasite that causes vivax malaria (MALARIA, VIVAX). This species is found almost everywhere malaria is endemic and is the only one that has a range extending into the temperate regions. Plasmodium vivaxs,vivax, Plasmodium
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000913 Antibodies, Protozoan Immunoglobulins produced in a response to PROTOZOAN ANTIGENS. Protozoan Antibodies
D014614 Vaccines, Synthetic Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified. Antigens, Synthetic,Chemical Vaccine,Chemical Vaccines,Immunogens, Synthetic,Molecular Vaccine,Molecular Vaccines,Recombinant Vaccine,Semisynthetic Vaccine,Semisynthetic Vaccines,Synthetic Antigen,Synthetic Vaccine,Synthetic Vaccines,Vaccines, Recombinant,Synthetic Antigens,Synthetic Immunogens,Vaccines, Chemical,Vaccines, Molecular,Vaccines, Semisynthetic,Antigen, Synthetic,Recombinant Vaccines,Vaccine, Chemical,Vaccine, Molecular,Vaccine, Recombinant,Vaccine, Semisynthetic,Vaccine, Synthetic

Related Publications

Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
March 1991, Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ),
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
May 2003, European journal of immunology,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
March 1995, Molecular medicine (Cambridge, Mass.),
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
January 1971, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
May 2009, Vaccine,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
September 2018, Antimicrobial agents and chemotherapy,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
March 2016, Malaria journal,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
December 2005, Comparative medicine,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
January 1978, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Angélica Castellanos, and Myriam Arévalo-Herrera, and Nora Restrepo, and Leonel Gulloso, and Giampietro Corradin, and Sócrates Herrera
June 1980, The Journal of parasitology,
Copied contents to your clipboard!